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| The Mechanism of NK4 Gene on Proliferation and Apoptosis in Non-Hodgkin Lymphoma Cells Based on Sirt1/p53 Signaling Pathway |
| BEN Haixiang, XU Juan |
| Rugao People's Hospital, Jiangsu Nantong 226500, China |
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Abstract Objective: To investigate the effect of NK4 gene on the proliferation and apoptosis of non-Hodgkin’s lymphoma cells. Methods: The expression levels of NK4 mRNA in NHL cell lines (Jurkat and Raji cells) and normal B cells (normal) were detected by qRT-PCR. pcDNA3.1-NK4 was transfected into Raji cells to increase the expression level of NK4. Cell proliferation was detected by CCK-8, apoptosis was detected by flow cytometry, and cell migration and invasion were detected by transwell assay to evaluate cell growth progress. Western blot was used to detect Sirt1/p53 signaling pathway-related proteins. Results: NK4 gene was lowly expressed in NHL cells. After up-regulating NK4 expression in NHL cell line Raji cells, the proliferation, migration and invasion ability of Raji cells decreased significantly, while the apoptosis rate increased. In addition, up-regulation of NK4 expression significantly decreased Sirt1 protein expression and increased p53 level. Addition of Sirt1 activator SRT1720 partially inhibited cell growth. Conclusion: NK4 gene mediated Sirt1/p53 signaling pathway inhibited proliferation and promoted apoptosis of non-Hodgkin lymphoma cells.
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2023, Vol. 29 
