基于AMPK-ACC信号通路探究miR-543对肺癌细胞放疗敏感性的机制研究

doi:10.3969/j.issn.1006-6233.2023.07.03

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Abstract Objective: To explore the potential role of miR-543 in regulating the radiosensitivity of NSCLC cells. Methods: The expression of miR-543 was detected by qRT-PCR; CCK8,colony formation assay and flow cytometry were used to explore the effect of miR-543 on the biological function of NSCLC cells.The expression of proteins related to AMPK-ACC signaling pathway was detected by WB.Reversion experiments and radiation treatment experiments were used to further verify the effect of miR-543 on the malignant progression and radiotherapy resistance of NSCLC cells through the AMPK-ACC signaling pathway. Results: miR-53 was up-regulated in NSCLC tissues and cells.Silencing miR-53 can inhibit the proliferation ability of NSCLC cells,promote cell cycle arrest and apoptosis,and weaken radiotherapy resistance.In addition,miR-53 is involved in the regulation of AMPK-ACC signaling pathway,and the use of compound C (an inhibitor of AMPK-ACC signaling pathway) can attenuate the effect of miR-53 silencing on NSCLC cell behavior and radiosensitivity. Conclusion: The results of this study suggest that miR-53 can enhance the radiotherapy resistance mechanism in NSCLC cells by regulating the AMPK-ACC signaling pathway,suggesting that targeting miR-53 may be a potential therapeutic target to improve the radiotherapy sensitivity in NSCLC.

Key words： miR-543 Non-small cell lung carcinoma Radiotherapy resistance AMPK-ACC pathway

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	Articles by authors
	CHU Xiangjian
	CAO Haiyan
	DAI Meiyun

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CHU Xiangjian,CAO Haiyan,DAI Meiyun. The Mechanism of miR-543 on Radiosensitivity in Lung Cancer Cells Based on AMPK-ACC Signaling Pathway[J]. HeBei Med, 2023, 29(7): 1068-1073.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.07.03 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I7/1068

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