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| Expressions of COX-2 Protein, C-erbB-2, P53 and VEGF-C Protein in Breast Cancer Tissues and Their Relationships with Clinicopathological Parameters and Prognosis |
| LI Ruiqing, ZHANG Jie |
| The Affiliated Hospital of Yangzhou University, Jiangsu Yangzhou 225000, China |
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Abstract Objective: To analyze the expressions of cyclooxygenase-2 (COX-2) protein, human epidermal growth factor receptor 2 (c-erbB-2), mutant P53 protein, and vascular endothelial growth factor c (VEGF-C) protein in breast cancer tissues and their relationships with clinicopathological parameters and prognosis. Methods: The surgical resection specimens of 103 patients with breast cancer who were treated in the hospital from March 2017 to May 2019 were selected. The protein positive rates of COX-2, c-erbB-2, P53 and VEGF-C were determined by immunohistochemistry. The relationships of levels of the above indicators with pathological parameters such as TNM staging and differentiation degree were compared. The survival data of patients at 3 years after surgery were followed up, and Kaplan-Meier method was used to analyze the effects of protein levels of COX-2, c-erbB-2, P53 and VEGF-C on the prognosis survival. Results: TNM staging and differentiation degree were correlated with positive COX-2 (P<0.05). Tumor diameter ≥3cm and lymph node metastasis were associated with positive c-erbB-2 (P<0.05). TNM staging, differentiation degree and lymph node metastasis were correlated with positive P53 (P<0.05). Lymph node metastasis was related to positive VEGF-C (P<0.05). 103 patients were followed up for 3 years, with a follow-up rate of 100%; 20 patients died (19.42%) and 83 patients survived (80.58%) as of May 2022; positive patients were divided into survival and death subgroups according to the death status, and the histochemical scores of patients in the death subgroup were significantly higher than those in the survival subgroup at enrollment (P<0.05). Log-rank χ2 test showed that there were statistical differences in the cumulative survival rates between patients with positive and negative P53 and VEGF-C after 3 years of follow-up (χ2=14.000, 5.206, all P<0.05). There were no statistically significant differences in the cumulative survival rates between patients with positive and negative COX-2 and c-erbB-2 (χ2=1.761, 2.830, P=0.184, 0.093). Conclusion: The protein positive rates of COX-2, c-erbB-2, P53 and VEGF-C in breast cancer tissues are related to TNM staging, lymph node metastasis, differentiation degree and other pathological parameters, and they have certain guiding significance on the evaluation of prognosis of patients.
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2023, Vol. 29 
