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| Proliferation and Osteogenic Differentiation of Rat BMSCs by Different Tension Stress |
| DENG Haiyan, GULIZAINU Yibulayin, SUN Jiangwei, et al |
| The Second Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi 830000, China |
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Abstract Objective: To examine the effects of the magnitude and duration of drafting stress on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: Ten SPF-grade SD rats were isolated and cultured and BMSCs were identified by osteogenic and lipogenic differentiation methods. Cell grouping was set for the magnitude of drafting stress loading (0.5% stretch, 5% stretch, 10% stretch, 15% stretch) and duration of action (0.5h, 6h, 12h, 24h, 48h, 72h). Proliferation of BMSCs was measured by CCK-8 method. The BCIP/NBT method was used to determine cellular alkaline phosphatase activity. Cellular osteogenic and lipogenic differentiation was detected by alizarin red and oil red O staining.Results: Tensile stress at 10% stretch for 12h maximally increased the proliferation rate of BMSCs by 1.53-fold, which was higher than that of the 5% and 15% stretch groups, with statistically significant differences (P<0.01). tensile stress at 5% stretch for 48h maximally increased alkaline phosphatase expression of BMSCs by 4.2-fold, increased mineralized nodule formation of BMSCs by 65%, and decreased BMSCs formation of lipid droplets by 32%, which was higher than the 10% and 15% stretch amplitude groups, with statistically significant differences (P<0.05 or P<0.01).Conclusion: The best effect of tensile stress stretching at 10% for 12h was observed in promoting the proliferation of BMSCs. Draft stress of 5% for 48h had the most significant effect on the formation of alkaline phosphatase and mineralized nodules in BMSCs, which in turn promoted osteogenic differentiation of BMSCs. The magnitude and duration of tension stress required to promote the proliferation and osteogenic differentiation of BMSCs varied.
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2023, Vol. 29 
