|
|
|
| Effects of Paeoniflorin on Wound Healing and HIF-1α/VEGF/VEGFR2 Signaling Pathway in Rats with Refractory Ulcers |
| WU Xi, CHEN Gao |
| Xiaogan Hospital Affiliated to Wuhan University of Science and Technology/Xiaogan Central Hospital, Hubei Xiaogan 432000, China |
|
|
|
|
Abstract Objective: To investigate the influence and mechanism of paeoniflorin (PF) on wound healing in rats with refractory ulcer by regulating hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF)/vascular endothelial growth factor receptor 2 (VEGFR2). Methods: Sixty SD rats were randomly grouped into Sham operation (Sham) group, Model group, PF group (30 mg/kg,twice a day), PX-478 group (100 mg/kg HIF-1α/VEGF/VEGFR2 pathway inhibitor PX-478,once a day), and PF+PX-478 group, 12 animals in each group, twice a day, for 2 weeks. The rat model of refractory wound was made by injecting hydrocortisone into the general wound surface. The camera combined with Image J software was used to calculate the wound healing rate of each group of rats; ELISA method was used to detect oxidative stress indicators in rat serum; HE staining was used to detect the pathological changes of rat wounds; TUNEL staining was used to detect apoptosis in wound tissue; RT-qPCR was used to detect the expressions of transforming growth factor β1 (TGF-β1) and platelet-derived factor (PDGF) in rat wound tissue; Western blotting was used to detect the expression of HIF-1α/VEGF/VEGFR2 pathway-related proteins in rat wound tissue. Results: Compared with the Sham group, the wound fibroblasts and capillaries of the rats in the Model group were sparse, the healing rate, levels of SOD, GSH-PX, mRNA levels of TGF-β1, PDGF, and protein levels of HIF-1α, VEGF, VEGFR2 were obviously decreased (P<0.05), the level of MDA and the apoptosis rate of wound tissue were obviously increased (P<0.05); compared with the Model group, the wound fibroblasts and capillaries of the rats in the PF group were denser (P<0.05), the healing rate, levels of SOD, GSH-PX, mRNA levels of TGF-β1, PDGF, and protein levels of HIF-1α, VEGF, VEGFR2 were obviously increased (P<0.05), the level of MDA and the apoptosis rate of wound tissue were obviously decreased (P<0.05), the corresponding indicators in the PX-478 group showed opposite trends (P<0.05); compared with the PF group, the wound fibroblasts and capillaries of the rats in the PF+PX-478 group were sparse (P<0.05), the healing rate, levels of SOD, GSH-PX, mRNA levels of TGF-β1, PDGF, and protein levels of HIF-1α, VEGF, VEGFR2 were obviously decreased (P<0.05), and the level of MDA and the apoptosis rate of wound tissue were obviously increased (P<0.05). Conclusion: PF may improve wound healing in rats with refractory ulcer by activating the HIF-1α/VEGF/VEGFR2 pathway.
|
|
|
|
|
|
[1] Salehi H,Mehrasa M,Nasri-Nasrabadi B,et al.Effects of nanozeolite/starch thermoplastic hydrogels on wound healing[J].Res Med Sci,2017,22(1):110-118.
[2] Larouche J,Sheoran S,Maruyama K.Martino MM.Immune regulation of skin wound healing:mechanisms and novel therapeutic targets[J].Adv Wound Care (New Rochelle),2018,7(7):209-231.
[3] Ma X,Zhang W,Jiang Y,et al.Paeoniflorin,a natural product with multiple targets in liver diseases-A mini review[J].Front Pharmacol,2020,11(1):531-537.
[4] Zhu Y,Wang Y,Jia Y,et al.Roxadustat promotes angiogenesis through HIF-1α/VEGF/VEGFR2 signaling and accelerates cutaneous wound healing in diabetic rats[J].Wound Repair Regen,2019,27(4):324-334.
[5] 朱小雨,贡翊斐,吴迎,等.十伤灵促进大鼠难愈性溃疡创面愈合的机制研究[J].现代中西医结合杂志,2021,30(21):2295-2298.
[6] Han G,Ceilley R.Chronic wound healing:a review of current management and treatments[J].Adv Ther,2017,34(3):599-610.
[7] Bilgen F,Ural A,Kurutas EB,et al.The effect of oxidative stress and raftlin levels on wound healing[J].Int Wound,2019,16(5):1178-1184.
[8] 吴遵秋,孔祥耀,黎小梅,等.有柄石韦对小鼠皮肤伤口愈合及转化生长因子-β1水平的影响[J].贵州医科大学学报,2020,45(11):1270-1274. |
|
|
|
2023, Vol. 29 
