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| Effects of Downregulation of SIP1 on Apoptosis Invasion and Migration of Endometrial Cancer Cells Based on ROCK-2/Moesin Signaling Pathway |
| LIU Xuejie, LI Xiaoyan, et al |
| Baoding Second Central Hospital, Hebei Baoding 072750, China |
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Abstract Objective: To investigate the effect of down-regulation of SIP1 on apoptosis, migration and invasion of endometrial cancer cells and its mechanism. Methods: Human endometrial carcinoma cell line HEC-1A was divided into control group, NC group, SIP1 down-regulated group (transfected with si-SIP1), Y27632 group (ROCK inhibitor), SIP1 down-regulated group+Y27632 group (transfected with si-SIP1+ROCK inhibitor). The apoptosis, migration ability and invasion ability of each group were detected by flow cytometry and Transwell assay. The expression levels of apoptosis key protein b-lymphoma-2 gene (Bcl-2), apoptosis gene Bcl-2 related protein X (Bax), caspase family protein 3 (caspase 3), Rho related kinase 2 (ROCK-2) and Moesin were detected by Western blot. Twenty SPF-grade BALB/c mice were tumor-loaded subcutaneously to establish an endometrial cancer tumor model. The control group (n=10) was tumor-loaded with human endometrial cancer HEC-1A cells, and the SIP1 down-regulated group (n=10) was tumor-loaded with human endometrial cancer HEC-1A cells with down-regulated SIP1. Tumor volume was measured with vernier caliper every 3 days, and the tumor growth curve was drawn for 21 days. At the end point, the tumor mass of each mouse was measured and the expression levels of ROCK-2 and Moesin protein in tumor tissues were detected. Results: The results of cell experiment showed that compared with the control group and NC group, the proportion of apoptosis, the number of cell migration and cell invasion in SIP1 down regulation group, Y27632 group and SIP1 down regulation + Y27632 group increased significantly (P<0.01), there was no significant difference between NC group and control group (P>0.05). The results of Western blot showed that the expression levels of ROCK-2 and Moesin in SIP1 down regulated group, Y27632 group and SIP1 down regulated + Y27632 group decreased compared with the control group and NC group (P<0.01). The mice experiment revealed that compared with the control group, the tumor volume, tumor mass and the expression levels of ROCK-2 and Moesin in tumor tissues decreased significantly after down-regulation of SIP1 (P<0.01). Conclusion: Down regulation of SIP1 can promote apoptosis, inhibit migration and invasion of human endometrial cancer cell line HEC-1A, and its may be related to the regulation of ROCK-2/Moesin signal pathway.
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2023, Vol. 29 
