circ_0086414靶向miR-155-5p抑制Hippo/YAP信号通路调控口腔鳞癌细胞增殖迁移和侵袭

doi:10.3969/j.issn.1006-6233.2023.03.02

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Abstract Objective: To investigate the effect of circ-0086414 targeting miR-155-5p on the inhibition of Hippo/Yap signaling pathway in regulating the proliferation, migration and invasion of oral squamous cell carcinoma cells. Methods: RT-PCR was used to detect the expression of circ-0086414 in HIOEC, Tca8113, CAL27 and SCC-9 cells.SCC-9 cells which were not transfected with any plasmid were set as control group; SCC-9 cells transfected with pcDNA-circ-0086414, pcDNA-NC, miR-155-5p inhibitor and miR-NC inhibitor plasmids were set as pcDNA-circ-0086414 group, pcDNA-NC group, miR-155-5p group and miR-NC group respectively; the pcDNA-circ-0086414 plasmid was transfected into SCC-9 cells, and 5 μmol/L Verteporfin was co-cultured and set as Verteporfin group. The expression of circ-0086414 and miR-155-5p was detected by RT-PCR; CCK-8 was used to detect cell proliferation; Transwell chamber method was used to detect the invasive ability of cells; the cell migration ability was detected by scratch test; the expression of Lats1, p-Lats1, YAP, p-YAP protein in cells was detected by Western blot; the target relationship between circ-0,086,414 and miR-155-5p was verified by double luciferase reporting system experiment. Results: Compared with human immortalized oral epithelial cells HIOECcirc-0086414 (1.00±0.10) and miR-155-5p (1.00±0.09), the expression of circ-0086414 (0.73±0.07), (0.51±0.05) and (0.36±0.03) in oral squamous cell carcinoma cell lines Tca8113, CAL27 and SCC-9 significantly decreased, and the expression of miR-155-5p (1.36±0.11), (1.57±0.13) and (1.89±0.15) significantly increased (P<0.05); the expression of circ-0086414 and miR-155-5p in SCC-9 cells changed most significantly (P<0.05). Compared with the control group, the expression of circ-0086414 in pcDNA-circ-0086414 group was significantly higher (1.26±0.12), and the expression of miR-155-5p in miR-155-5p group was significantly lower (0.73±0.07) (P<0.001). Compared with the control group, the proliferation, invasion (123.56±5.87) and migration (16.90±1.62) of cells in pcDNA-circ-0086414 group significantly decreased (P<0.05). Compared with pcDNA-circ-0086414 group, the proliferation, invasion and migration of cells in Verteporfin group significantly increased (P<0.05). Compared with the control group, the expression of Last1 (1.12±0.10), p-Last1 (1.49±0.12) and p-YAP (1.4±0.11) protein in pcDNA-circ-0086414 group significantly increased, while the expression of YAP protein (0.45±0.04) significantly decreased (P<0.001). Compared with pcDNA-circ-0086414 group, the expression of Last1 (0.61±0.06), p-Last1 (0.82±0.08) and p-YAP protein (0.58±0.05) in the cells of Verteporfin group significantly decreased, and the expression of YAP protein (0.93±0.09) significantly increased (P<0.001). The luciferase activity of wild-type circ-0086414 (circ-0086414-WT) miR-155-5p group (0.26±0.03) was significantly lower than that of miR-NC group (P<0.05). Conclusion: Overexpression of circ-0086414 can inhibit the proliferation, invasion and migration of oral squamous cell carcinoma SCC-9 cells. Its mechanism is related to inhibition of miR-155-5p expression and activation of Hippo Yap signal pathway.

Key words： Circ-0086414 miR-155-5p Hippo/Yap signal pathway Oral squamous cell carcinoma Proliferation Attack Transfer

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	LI Ling
	SONG Qingqing
	BI Ke

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LI Ling,SONG Qingqing,BI Ke. circ_0086414 Targeting miR-155-5p Inhibits Hippo/YAP Signaling Pathway to Regulate Proliferation Migration and Invasion of Oral Squamous Cell Carcinoma Cells[J]. HeBei Med, 2023, 29(3): 358-364.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.03.02 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I3/358

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