LINC00342调控miR-384对肺鳞癌细胞增殖侵袭迁移的影响研究

doi:10.3969/j.issn.1006-6233.2023.02.01

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Abstract Objective: To investigate the influences of LINC00342 on the proliferation, invasion, and migration of lung squamous cell carcinoma through miR-384. Methods: Lung squamous cell carcinoma SK-MES-1 cells were grouped into the ctrl group (normally cultured SK-MES-1 cells), si-NC group (transfected with si-NC), and si-LINC00342 group (transfected with si-LINC00342), si-LINC00342+inhibitor-NC group (co-transfected with si-LINC00342 and inhibitor-NC), and si-LINC00342+ miR-384 inhibitor group (co-transfected with si-LINC00342 and miR-384 inhibitor); RT-qPCR was applied to detect the expression of LINC00342 and miR-384 in cells; CCK-8 assay and Transwell chamber were applied to detect cell proliferation and invasion; scratch assay and flow cytometry were used to cell migration and apoptosis; Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-2 (MMP-2), MMP-9, and cysteine protease-3 (caspase-3) in cells; and dual-luciferase reporter assay was used to verify the relationship between LINC00342 and miR-384. Results: Compared with the ctrl group and si-NC group, the OD450 value, the number of cell invasions, the wound healing rate, the expressions of PCNA, MMP-2, and MMP-9 in SK-MES-1 cells in the si-LINC00342 group was obviously decreased, and the cell apoptosis was obviously decreased, the mortality rate and the expression of caspase-3 were obviously increased (P<0.05); inhibiting the expression of miR-384 attenuated the inhibitory effect of knockdown of LINC00342 on the proliferation, invasion, and migration of SK-MES-1 cells, and reduced the apoptosis ability; LINC00342 targeted miR-384 expression. Conclusion: Knockdown of LINC00342 may inhibit the proliferation, invasion, and migration of SK-MES-1 cells by targeting miR-384.

Key words： LINC00342 miR-384 Lung squamous cell carcinoma Proliferation Invasion Migration

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	XU Yueliang
	WANG Xiaobin
	YANG Yaoqing

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XU Yueliang,WANG Xiaobin,YANG Yaoqing. LINC00342 Regulates the Effect of miR-384 on the Proliferation Invasion and Migration of Lung Squamous Carcinoma Cells[J]. HeBei Med, 2023, 29(2): 177-182.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.02.01 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I2/177

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