卵巢癌组织中circKIF4A和miR-942-5p的表达及其与患者临床病理特征及预后的关系

doi:10.3969/j.issn.1006-6233.2023.01.024

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Abstract Objective: To investigate the expressions of miR-942-5p and circKIF4A in ovarian cancer tissues and their relationship with the prognosis of patients. Methods: A total of 154 ovarian cancer patients who came to our hospital for surgical treatment from January 2015 to January 2017 were collected as the research subjects. The cancer tissues and adjacent tissues of the surgical patients were collected, and the relationship between the expressions of miR-942-5p and circKIF4A with clinicopathological characteristics and prognosis was retrospectively analyzed. The levels of Mir-942-5p and circKIF4A were detected by real-time fluorescence quantitative PCR (QRT-PCR); Kaplan-Meier method was used to analyze the relationship between the expression of different miR-942-5p and circKIF4A and the survival time of patients; multivariate Cox regression risk model was used to analyze the prognostic factors. Results: The expression of circKIF4A was higher in cancer tissues of ovarian cancer patients (4.27±0.33) compared with paraneoplastic tissues (1.00±0.05), and miR-942-5p expression was lower (0.63±0.12) compared with paraneoplastic tissues (1.01±0.19), with a negative correlation between the two expressions (r=-0.312, P<0.05); CircKIF4A and miR-942-5p expression in cancer tissues of ovarian cancer patients was not associated with age, pathological type, WHO histological grade, tumor diameter and degree of infiltration, but with degree of differentiation, lymph node metastasis, TNM stage, tumor grade and presence of ascites (P<0.05); Kaplan-Meier analysis showed that the 5-year cumulative survival rate of patients in the circKIF4A high expression group was significantly lower at 26.92% (21/78) than that of patients in the low expression group at 47.37% (36/76), with a statistically significant difference (Log rank χ²=6.651, P=0.010); The 5-year cumulative survival rate of patients in the miR-942-5p high expression group was significantly higher at 46.25% (37/80) than that of patients in the low expression group at 27.03% (20/74), with a statistically significant difference (Log rank χ²=9.978, P=0.000); Cox results showed that lymph node metastasis, TNM stage, degree of differentiation, high expression of circKIF4A, and miR-942-5p low expression were all risk factors affecting the survival status of ovarian cancer patients. Conclusion: The expression of miR-942-5p was low and circKIF4A was high in cancer tissues of ovarian cancer patients, and the expression of both was negatively correlated with the disease development and prognosis of ovarian cancer patients.

Key words： Ovarian cancer CircKIF4A miR-942-5p Pathological features Prognosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2023.01.024 OR http://www.hbyxzzs.cn/EN/Y2023/V29/I1/126

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