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| Bilobalide Regulates SOCS2 to Inhibit the Expression of Inflammatory Fibrotic Components in High Glucose-Induced Glomerular Mesangial Cells |
| LI Xiaoyan, MO Liwen, CHENG Ying, et al |
| General Hospital of Western Theater, Sichuan Chengdu 610031, China |
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Abstract Objective: To investigate whether bilobalide affects the expression of inflammatory and fibrotic components in high glucose-induced glomerular mesangial cells by regulating suppressor of cytokine signaling 2 (SOCS2). Methods: Human mesangial cells (HMC) were divided into the following groups:NG group (normal culture),HG group (30 mmoL/L glucose),HG+ low,medium and high bilobalide groups (6μmoL/L,12μmoL/L,24μmoL/L bilobalide and 30 mmoL/L glucose),HG+high bilobalide+NC group (transfected small interfering RNA negative control,24μmoL/L bilobalide and 30 mmoL/L glucose) and HG+high bilobalide+interfering SOCS2 group (transfected with small interfering RNA against SOCS2,24μmoL/L bilobalide and 30 mmoL/L glucose). Western blotting was used to detect the protein expressions of SOCS2 and fibrotic components transforming growth factor-β1 (TGF-β1),connective tissue growth factor (CTGF),and fibronectin (FN). Enzyme-linked immunosorbent assay (ELISA) was used to detect inflammation factors tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expression. Results: Compared with the NG group,the protein expressions of TGF-β1[(0. 82±0. 04) vs (0. 23±0. 02)],CTGF[(0. 69±0. 05) vs (0. 12±0. 01)],FN[(0. 81±0. 06) vs (0. 19±0. 03)],TNF-α [(580. 58±41. 78) vs (148. 46±11. 47)] pg/mL and IL-6 [(427. 82±31. 51) vs (60. 72±6. 24)] pg/mL in the glomerular HMC cells of the HG group were increased,while the protein expression of SOCS2 [(0. 22±0. 02) vs (0. 79±0. 06)] was decreased (P<0. 05); compared with the HG group,the protein expressions of TGF-β1,CTGF and FN,and the expression levels of TNF-α and IL-6 in the glomerular mesangial HMC cells of the HG+ low,medium and high bilobalide groups gradually decreased,but the protein expression of SOCS2 gradually increased (P<0. 05),and all in a concentration-dependent manner. Compared with HG+high bilobalide+NC group,the expression of SOCS2 protein in glomerular mesangial HMC cells in HG+high bilobalide+interfering SOCS2 group decreased,the expression of TGF-β1,CTGF,FN and TNF-α,IL-6 was increased (P<0. 05). Conclusion: Bilobalide can inhibit the expression of inflammatory and fibrotic components in mesangial cells induced by high glucose by up-regulating the expression of SOCS2.
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2022, Vol. 28 
