结直肠癌组织中lncRNA TUSC7与miR-1270表达的相关性及临床病理意义

doi:10.3969/j.issn.1006-6233.2022.10.08

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Abstract Objective: To investigate the correlation between long non-coding RNA (lncRNA) tumor suppressor candidate 7 (TUSC7) and microRNA-1270 (miR-1270) in colorectal cancer (CRC) tissues and their relationship with the prognosis of CRC patients. Methods: The lnCAR database was used to search the expression of lncRNA TUSC7 in CRC and normal colorectal tissues.A total of 130 CRC patients accepted by our hospital from June 2018 to January 2019 were regarded as the research subjects.The surgically resected CRC and paracancerous tissues were collected,and the expression levels of lncRNA TUSC7 and miR-1270 in CRC and paracancerous tissues were detected; the correlation between the expression level of lncRNA TUSC7 and miR-1270 in CRC tissues was analyzed; the relationship between the expression levels of lncRNA TUSC7 and miR-1270 in CRC tissues and the clinicopathological characteristics and prognosis of CRC were analyzed,Bioinformatics website was used to predict the relationship between lncRNA TUSC7 and miR-1270. Results: The lnCAR database analysis showed that the expression level of lncRNA TUSC7 in CRC tissue was lower than that in normal colorectal tissue (P<0.05); qRT-PCR results showed that the expression level of lncRNA TUSC7 in CRC tissue was lower than that in adjacent tissue (P<0.05),and the expression level of miR-1270 was higher than that in adjacent tissue (P<0.05); the expression levels of lncRNA TUSC7 and miR-1270 in CRC tissue were correlated with the degree of differentiation,lymph node metastasis,depth of invasion and TNM stage (P<0.05); bioinformatics analysis showed that lncRNA TUSC7 had a targeted binding site for miR-1270; the expression level of lncRNA TUSC7 in CRC tissue was negatively correlated with miR-1270 (r=-0.578,P<0.05); the cumulative survival rate of CRC patients in lncRNA TUSC7 low expression group and miR-1270 high expression group was lower than that in lncRNA TUSC7 high expression group and miR-1270 low expression group (P<0.05). Conclusion: The expression of lncRNA TUSC7 is down-regulated and the expression of miR-1270 is up-regulated in CRC tissue,and the two are negatively correlated,which provides a new marker for clinical diagnosis and treatment of CRC and evaluation of the prognosis of CRC patients.

Key words： MicroRNA-1270 Colorectal cancer Long non-coding RNA tumor suppressor candidate 7

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.10.08 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I10/1625

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