Survivin通过Wnt/β-catenin信号通路调控食管癌的侵袭和迁移的机制研究

doi:10.3969/j.issn.1006-6233.2022.10.07

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Abstract Objective: To investigate the expression of Survivin in esophageal cancer and its effect on Wnt/β-catenin signaling pathway,invasion and migration in esophageal cancer cells. Methods: The expression of Survivin protein in human esophageal cancer tissues and EC109 cells was examined and its correlation with clinical case characteristics was analyzed.Survivin small interfering RNA was transfected into oesophageal cancer EC109 cells by liposome mediated method.Relative expression of gene mRNA was detected by RT-qPCR.Protein expression was detected by immunoprotein blotting (Western blot).Cell viability,migration and invasion ability were measured by MTT,scratch healing and Transwell assays. Results: The expression of Survivin was increased in esophageal cancer tissues and EC109 cells.The TNM stage,differentiation and lymph node metastasis of esophageal cancer tissues correlated with positive Survivin expression; down-regulation of Survivin by RNA interference significantly inhibited Survivin mRNA and protein expression in esophageal cancer cells.The expression of wnt3α,β-catenin,cyclinD1 and c-myc in esophageal cancer cells decreased after down-regulation of Survivin,and the Wnt/β-catenin signaling pathway was inhibited,and the survival,migration and invasion ability of esophageal cancer cells were suppressed. Conclusion: Down-regulation of Survivin gene can inhibit the survival,migration and invasion of esophageal cancer cells by affecting the Wnt/β-catenin signaling pathway.

Key words： RNA interference Survivin Wnt/β-catenin signal pathway Esophageal cancer

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.10.07 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I10/1620

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