|
|
|
| Effect of Adipose-Derived Stem Cells on Knee Osteoarthritis in Mice |
| BAI Bing, SU Mingxia, SUN Yanbao, et al |
| Chengde Central Hospital, Hebei Chengde 067000, China |
|
|
|
|
Abstract Objective: To investigate the effects and possible mechanisms of suprapatellar-derived ASC autograft on knee inflammation and degenerative grade of cartilage in a mouse KOA model. Methods: ASC in the suprapatellar or infrapatellar region were isolated, ASC proliferation and fat-forming, osteogenic and cartilage-forming directed differentiation ability were measured respectively, the OA mouse model was replicated and cell transplantation was performed, and the GAG analysis and injury score of mouse knee tissue were examined. Results: In vitro experiments showed a significant increase in fat formation, osteogenesis and cartilage formation in the suprapatellar-derived ASC group compared to the infrapatellar-derived and infrapatellar-differentiated cell groups. In vivo experiments showed a significant increase in knee dimensions in all three groups after OA induction. hASC, SVF and PRP intervention groups all showed a significant decrease in increased knee volume compared to the control group, a significant increase in GAG content and a significant decrease in OA injury score in the hASC group. Conclusion: Human suprapatellar fat pad-derived ASC promotes cartilage regeneration by promoting effective endogenous cartilage formation in the knee joint in KOA mice.
|
|
|
|
|
|
[1] Mobasheri A and Batt M.An update on the pathophysiology of osteoarthritis[J].Ann Phys Rehabil Med,2016,59(5-6):333-339.
[2] Vega A,Martin-Ferrero MA,Del Canto F,et al.Treatment of knee osteoarthritis with allogeneic bone marrow mesenchymal stem cells: a randomized controlled trial[J].Transplantation,2015,99(8):1681-1690.
[3] Koh YG and Choi YJ.Infrapatellar fat pad-derived mesenchymal stem cell therapy for knee osteoarthritis[J].Knee,2012,19(6):902-907.
[4] Pires de Carvalho P,Hamel KM,Duarte R,et al.Comparison of infrapatellar and subcutaneous adipose tissue stromal vascular fraction and stromal/stem cells in osteoarthritic subjects[J].Tissue Eng Regen Med,2014,8(10):757-762.
[5] Ballegaard C,Riis RG,Bliddal H,et al.Knee pain and inflammation in the infrapatellar fat pad estimated by conventional and dynamic contrast-enhanced magnetic resonance imaging in obese patients with osteoarthritis: a cross-sectional study[J].Osteoarthritis Cartilage,2014,22(7):933-940.
[6] Eymard F,Pigenet A,Citadelle D,et al.Induction of an inflammatory and prodegradative phenotype in autologous fibroblast-like synoviocytes by the infrapatellar fat pad from patients with knee osteoarthritis[J].Arthritis Rheumatol,2014,66(8):2165-2174.
[7] Napolitano M,Matera S,Bossio M,et al.Autologous platelet gel for tissue regeneration in degenerative disorders of the knee[J].Blood Transfus,2012,10(1):72-77.
[8] Meheux CJ,McCulloch PC,Lintner DM,et al.Efficacy of intra-articular platelet-rich plasma injections in knee osteoarthritis: a systematic review[J].Arthroscopy,2016,32(3):495-505.
[9] Pers YM,Rackwitz L,Ferreira R,et al.Adipose mesenchymal stromal cell-based therapy for severe osteoarthritis of the knee: a phase I dose-escalation trial[J].Stem Cells Transl Med,2016,5(7):847-856.
[10] Jadvar H,Desai B,Conti PS.Sodium 18F-fluoride PET/CT of bone,joint,and other disorders[J].Semin Nucl Med,2015,45(1):58-65.
[11] Brandl A,Angele P,Roll C,et al.Influence of the growth factors PDGF-BB,TGF-beta1 and bFGF on the replicative aging of human articular chondrocytes during in vitro expansion[J].Orthop Res,2010,28(3):354-360.
[12] Xie X,Zhang C,Tuan RS.Biology of platelet-rich plasma and its clinical application in cartilage repair[J].Arthritis Res Ther,2014,16(1):204.
[13] El Backly RM,Zaky SH,Canciani B,et al.Platelet rich plasma enhances osteoconductive properties of a hydroxyapatite-beta-tricalcium phosphate scaffold (Skelite) for late healing of critical size rabbit calvarial defects[J].Craniomaxillofac Surg,2014,42(5):70-79. |
|
|
|
2022, Vol. 28 
