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| Study of the Regulatory Mechanism of the Interaction Between Numb1 and ITGB1 in Gastric Cancer Progression and Drug Resistance |
| CHEN Yong, HE Donglei, ZHOU Jianghao, et al |
| The First Affiliated Hospital of Hainan Medical College, Hainan Haikou 570102, China |
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Abstract Objective: To investigate the interaction between cohesive protein 1 (Numb1) and integrin β1 (ITGB1) on gastric cancer progression and drug resistance and its related mechanisms. Methods: The expression levels of Numb1 and ITGB1 in tumors and adjacent tissues of 21 patients with gastric cancer were detected by qRT-PCR, and the correlation between the expression levels of Numb1 and ITGB1 was analyzed. The overexpressed vectors (flag-Numb1, flag-ITGB1, and flag-NC) were transfected into BGC-823 cells by lentivirus, and the binding sites of Numb1 and ITGB1 were detected by the immunoprecipitation method. si-NC, si-Numb1, and si-ITGB1 were transfected into BGC-823 cells. The mRNA expression levels of Numb1 and ITGB1 were detected by qRT-PCR and the protein expression levels of Numb1 and ITGB1 were detected by Western blot. CCK8 detected cell proliferation and sensitivity to oxaliplatin, cell migration was detected by cell scratch assay, and cell invasion was detected by Transwell. Results: The expression levels of Numb1 and ITGB1 were elevated in tumor tissues compared to paraneoplastic tissues (P<0.05), and Numb1 was positively correlated with ITGB1 expression levels (r=0.6270). In cellular assays, ITGB1 protein was detected in the immunoprecipitation products of flags overexpressing flag-Numb1, and Numb1 protein was detected in the immunoprecipitation products of flags overexpressing flag-ITGB1. Compared with the si-NC group, the si-Numb1 group showed reduced levels of Numb1 and ITGB1 mRNA and protein expression (P<0.05), reduced cell proliferation (P<0.05), increased sensitivity to oxaliplatin (P<0.05), and reduced cell migration and invasion (P<0.05). Compared with the si-NC group, the si-ITGB1 group showed reduced ITGB1 mRNA and protein expression levels (P<0.05), reduced cell proliferation (P<0.05), increased sensitivity to oxaliplatin (P<0.05) and reduced cell migration and invasion (P<0.05). Conclusion: Numb1 regulates ITGB1 expression levels through interaction with ITGB1 and promotes proliferation, migration, invasion and resistance to chemotherapeutic agents in gastric cancer cells
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2022, Vol. 28 
