血清miR-483-5p miR-487b表达与脓毒症所致急性肺损伤患者炎性因子病情和预后的关系研究

doi:10.3969/j.issn.1006-6233.2022.09.015

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Abstract Objective: To investigate the relationship between miR-483-5p and miR-487b and inflammatory factors, disease condition, and prognosis in patients with acute lung injury (ALI) induced by sepsis. Methods: A total of 133 sepsis patients admitted to the ICU of our hospital from September 2018 to December 2021 were selected, including 51 cases complicated with ALI (ALI group) and 82 cases without ALI (NALI group). According to oxygenation index [oxygen partial pressure/inhaled oxygen concentration], ALI group was divided into mild group (201 ~ 300mmHg) (16 cases), moderate group (101 ~ 200mmHg) (20 cases) and severe group (≤100mmHg) (15 cases). According to the 28-day survival status, the ALI group was divided into the survival group (28 cases) and the death group (23 cases). Another 43 volunteers were selected as the control group. The serum expressions of mir-483-5p and mir-487b and the levels of inflammatory factors in different groups were compared. Pearson analyzed the correlation between the expressions of miR-483-5p and miR-487b and the levels of inflammatory factors in patients with sepsis complicated with ALI. Receiver operating characteristic curve (ROC) analysis of the prognostic value of miR-483-5p and miR-487b in patients with sepsis-induced ALI. Results: The expression of miR-483-5P, the levels of interleukin-6 (IL-6), tumor necrosis factor -α (TNF-α), and interleukin-33 (IL-33) on the baseline in the ALI group were higher than those in NALI group and control group (P<0.05), and the expression of miR-487b on the baseline was lower than those in NALI group and control group (P<0.05). The expression of miR-483-5p, IL-6, TNF-α, and IL-33 on the baseline, 3d hospitalization, and 7d hospitalization in the severe group were higher than those in the moderate and mild groups (P<0.05), and the expression of miR-487b was lower than those in the moderate and mild groups (P<0.05). Serum miR-483-5p expression, IL-6, TNF-α, IL-33 levels on the baseline, 3d hospitalization, and 7d hospitalization in the death group were higher than those in the survival group (P<0.05), and miR-487b expression was lower than those in the survival group (P<0.05). The expression of miR-483-5P was positively correlated with the levels of IL-6, TNF-α, and IL-33 on the baseline, 3d hospitalization, and 7d hospitalization in patients with sepsis (P<0.05). The expression of miR-487b was negatively correlated with the levels of IL-6, TNF-α, and IL-33 on the baseline, 3d hospitalization, and 7d hospitalization (P<0.05). The area under the curve predicted by the combination of miR-483-5p and miR-487b on 7d hospitalization was 0.964, which was higher than those predicted by miR-483-5p and miR-487b alone (z=2.612, 3.141,P<0.05). Conclusion: The expression of miR-483-5p is up-regulated and miR-487b is down-regulated in serum of ALI patients induced by sepsis, which is related to excessive inflammatory response, aggravation of the disease, and poor prognosis.

Key words： Sepsis Acute lung injury Inflammatory factors Prognosis Micro ribonucleic acid MiR-483-5p MiR-487b

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.09.015 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I9/1484

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