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| Changes in the Behavioristics of IFN-α Induced Depression in Rats and in the Expression Level of 5-HTA2R in the CAl Region of Hippocampal Tissue |
| XU Baiyu, et al |
| Chengde Medical University, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the mechanism of depression by examining the behavioural and hippocampal histology and its 5-HTA2R expression levels in different ways of preparing rat depression models. Methods: Thirty rats were selected and divided by random number method into normal group,interferon group and CUMS model group,10 rats each.The rat depression model was prepared by subcutaneous injection of IFN-α for 12 weeks,and the traditional CUMS rat depression model was used as a control for a comparative study.Behavioural indicators such as body weight,results of the absenteeism test,sugar-water preference test and the time of feeding inhibition in the new environment were recorded at different times of the intervention; histological changes in the hippocampus of rats were observed by HE staining and auxin staining; changes in the expression level of 5-HTA2R in rats were detected by immunohistochemistry. Results: Compared with the control group,there was a statistical difference between the interferon group and the model group in terms of rat body weight and various behavioral experiment scores (P<0.05); compared with the model group,the duration of the new environmental inhibition of feeding was significantly longer in the interferon group,and there was a statistical difference (P<0.05),but there was no statistical difference (P>0.05) in the rat body weight change,the score of the absenteeism test and the results of the sugar-water preference experiment.There was a statistically significant difference (P<0.05) when the duration of the intervention was prolonged,with a gradual decrease in body weight,scores in the sugar-water preference test and the score of the absenteeism test,and a gradual increase of the duration of inhibition of feeding in the new environment (P>0.05).Both HE staining and auxin staining showed that the hippocampal CAl region of the model group showed obvious signs of injury,and the model group showed obvious signs of injury,with reduced cell numbers,increased cell gaps,fewer cell layers,sparse,disorganized,incomplete cell structure,and atrophy,with some cells showing vacuolation,smaller nuclei,solidified,and also visible swollen,lightly stained,fragmented,or even lost nuclei,showing eosinophilic staining.The interferon group was similar to the model group; the number of 5-HTA2R immunoreactive positive neurons in the hippocampal CAl region was reduced in the interferon group compared to the normal group,with a significant difference (71.6% vs 56.3%,χ2=4.85 P<0.05),and the number of 5-HTA2R receptor immunoreactive positive neurons in the hippocampal CAl region was slightly reduced compared to the model control group,but not statistically different (52.6% vs 56.3%,χ2=0.32 P>0.05). Conclusion: The ability of interferon to reduce the level of 5-HTA2R expression in the CAl region of the hippocampus may lead to depression in rats,providing a theoretical basis for the development of strategies for the preparation of depression models.
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2022, Vol. 28 
