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| Effect of Allicin Modulation of PI3K/Akt/Nrf2 Pathway on Endometriosis in Rats |
| DONG Xiujuan, CAO Ruoyu, YU Hongji, et al |
| Cangzhou Central Hospital, Hebei Cangzhou 061000, China |
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Abstract Objective: To investigate the effect of Allicin on endometriosis (EMs) in rats and its mechanism. Methods: The EMs rat model was prepared by auto-internal membrane transplantation, and the 30 model rats were randomly divided into model group, Allicin (10mg/kg) group and Allicin (10mg/kg) + LY294002 (PI3K specific inhibitor, 40mg/kg) group, n=10; another 10 rats were set as the sham operation group. The drugs were given once daily by intraperitoneal injection in each group (the rats in sham operation group and model group were given normal saline) for 4 weeks. The adhesion scores of endometriosis lesions was detected, the lesion volume was measured by calipers, the endometrium pathological examination was examed by HE staining method, the content of TNF-α, IL-1β, IL-6, IFN-γ were detected by ELISA method, the content of MDA and the activity of MPO, SOD, CAT in endometriosis lesions were detected by biochemical analysis method; the expression of Akt, p-Akt, Nrf2, HO-1, NF-κB were detected by Western blot method.Results: Compared with the sham operation group, the interstitium of endometriosis lesions in the model group is dense, blood vessels are proliferated and inflammatory cell infiltration changes. The contents of TNF-α, IL-1β, IL-6, IFN-γ, MDA in the ectopic lesion tissue were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1 were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01). Compared with the model group, the lesion volume and adhesion score were significantly decreased in Allicin group and Allicin + LY294002 group (P<0.05 or P<0.01); ectopic lesions include epithelial cell shedding, interstitial porosity, neovascularization; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were decreased while the activities of MPO, SOD, CAT were increased (P<0.05 or P<0.01); the expression of p-Akt, Nrf2, HO-1 were up-regulated and the ratio of p-Akt/Akt was increased (P<0.01), while the expression of NF-κB was down-regulated (P<0.01). Compared with the Allicin group, the lesion volume and adhesion score were significantly increased in Allicin + LY294002 group (P<0.01), the angiogenesis in ectopic lesions was increased; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1, NF-κB were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01).Conclusion: Allicin has a certain therapeutic effect on EMs in rats; which mechanism may be related to the activation of PI3K/Akt/Nrf2 pathway, thereby inhibiting inflammation and oxidative stress.
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2022, Vol. 28 
