乳腺癌组织中miR-4732-5p TSPAN13 mRNA的表达及临床意义

doi:10.3969/j.issn.1006-6233.2022.04.016

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Abstract Objective: To investigate the expression and clinical significance of microRNA (miRNA)-4732-5p and tetraspanin 13 (TSPAN13) mRNA in breast cancer tissues. Methods: Surgical specimens of 105 breast cancer patients hospitalized in our hospital from January 2017 to August 2018 were collected. QRT-PCR was performed to detect miR-4732-5p and TSPAN13 mRNA expression in cancer and paracancerous tissues. Pearson correlation analysis was used to aalyze the correlation between miR-4732-5p and TSPAN13 mRNA expression in breast cancer tissues, and their correlation with the clinicopathologic features. The K-M method was used to plot the survival curves of breast cancer patients with different miR-4732-5p and TSPAN13 mRNA expression, Multi-factor Cox regression analysis to analyze factors influencing poor prognosis in breast cancer patients.Results: Compared with paraneoplastic tissues, miR-4732-5p expression (0.629±0.169 vs. 1.131±0.273) was elevated and TSPAN13 mRNA expression (0.529±0.081 vs. 0.355±0.078) was decreased in breast cancer tissues, with statistical differences (t=16.019, -15.790, both P<0.001). Pearson correlation analysis showed a negative correlation between miR-4732-5p and TSPAN13 mRNA expression in breast cancer tissues (r=-0.732, P<0.001). The expression of miR-4732-5p and TSPAN13 mRNA was associated with the degree of breast cancer differentiation, TNM stage, and lymph node metastasis (P<0.05 ). At 3 years follow-up, a median of 28 months, the cumulative survival rate of 105 breast cancer patients was 87.62% (92/105). The cumulative survival rate of the high miR-4732-5p expression group was lower than that of the low miR-4732-5p expression group, and the cumulative survival rate of the high TSPAN13 mRNA expression group was higher than that of the low TSPAN13 mRNA expression group (P<0.05). Multi-factorial Cox regression analysis showed that TNM stage III-IV (HR=3.381, 95% CI: 1.658 to 5.377, P=0.018), lymph node metastasis (HR=4.546, 95% CI: 1.870 to 7.758, P=0.011), miR-4732-5p≥1.131 (HR=2.932. 95% CI: 1.482 to 4.770, P=0.015) were independent risk factors for poor prognosis and TSPAN13 mRNA ≥0.355 (HR=0.310, 95% CI: 0.156 to 0.729, P=0.044) were independent protective factors for breast cancer patients.Conclusion: Upregulation of miR-4732-5p expression and downregulation of TSPAN13 mRNA expression in breast cancer tissues correlate with differentiation, TNM stage and lymph node metastasis and may be a prognostic assessment biomarker for breast cancer patients.

Key words： Breast cancer MicroRNA-4732-5p Tetraspanin 13

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	XU Fan
	SHEN Yan
	ZHANG Xia

Cite this article:

XU Fan,SHEN Yan,ZHANG Xia. Expression and Clinical Significance of MiR-4732-5p and TSPAN13 MRNA in Breast Cancer Tissues[J]. HeBei Med, 2022, 28(4): 608-613.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.04.016 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I4/608

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