骨髓间充质干细胞外泌体miR-25-3p调控骨肉瘤细胞增殖迁移及侵袭能力的功能与机制研究

doi:10.3969/j.issn.1006-6233.2022.04.01

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Abstract Objective: To explore the effect and mechanism of bone marrow mesenchymal stem cell exosomal miR-25-3p on the proliferation, migration and invasion of osteosarcoma cells.Methods: Bone marrow MSCs were identified by flow cytometry, bone marrow MSC exosomes (BMSC exo) were extracted and identified, miR-NC and miR-25-3p mimic were transfected into MG-63 cells (miR-NC and miR-25-3p mimic groups), 10 μg/ml BMSC exo and an equal volume of PBS were incubated with MG-63 cells for 48h (BMSC exo group and PBS group), and exosomes secreted by BMSC in the miR-NC group and miR-25-3p mimic group (miR-NC exo and miR-25-3p exo) were extracted and co-incubated with MG-63 cells for 48h (miR-NC exo group and miR-25-3p exo group). MiR -25-3p exo group MG-63 cells were transfected with PTEN plasmid (miR-25-3p exo+PTEN group). qRT-PCR was performed to detect miR-25-3p expression, CCK8 assay to detect the proliferation ability of each group, Transwell assay to detect cell migration and invasion ability, dual luciferase reporter gene to detect miR-25-3p and PTEN targeting, Western blot for PTEN expression in each cell group.Results: BMSCs expressed their marker proteins CD29 and CD90, and the number of proliferation, migration and invasion of MG-63 cells was significantly increased in the miR-25-3p mimic group compared to the miR-NC group (P<0.05). Compared with the PBS group, miR-25-3p expression was significantly increased in MG-63 cells in the BMSC exo group (P<0.001), and cell proliferation, migration and invasion numbers were significantly increased (P<0.05). PTEN was the target gene of miR-25-3p, and compared with the miR-NC exo group, miR-25-3p exo group MG-63 cells miR-25-3p expression was significantly increased (P<0.05). 25-3p expression was significantly increased (P<0.001) and cell proliferation, migration and invasion numbers were significantly increased (P<0.05) in the miR-25-3p exo group compared to the miR-25-3p exo group, and cell proliferation, migration and invasion numbers were significantly decreased (P<0.05) in the miR-25-3p exo+PTEN group.Conclusion: Bone marrow mesenchymal stem cell exosome miR-25-3p promotes proliferation, migration and invasion of osteosarcoma by targeting and inhibiting PTEN.

Key words： Bone marrow mesenchymal stem cells MiR-25-3p Osteosarcoma cell

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	LIU Hantao
	ZHAO Lianghu
	QIN Hongmin

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LIU Hantao,ZHAO Lianghu,QIN Hongmin. The Function and Mechanism of the Bone Marrow Mesenchymal Stem Cell Exosome MiR-25-3p in Regulating the Proliferation Migration and Invasive Ability of Osteosarcoma Cells[J]. HeBei Med, 2022, 28(4): 529-533.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.04.01 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I4/529

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