人参皂苷Rg3调控Dickkopf相关蛋白3对肝癌细胞增殖和凋亡的影响

doi:10.3969/j.issn.1006-6233.2022.01.03

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Abstract Objective: To explore the effect of ginsenoside Rg3 on the proliferation of liver cancer cells and its mechanism. Methods: The human liver cancer cell line Hep G2 was used as the research subject which was divided into control group (Control), ginsenoside Rg3 group (G-Rg3), ginsenoside Rg3+Dickkopf-related protein 3 irrelevant fragment group (G-Rg3+DKK3-NC), and ginsenoside Rg3+Dickkopf-related protein siRNA group (G -Rg3+DKK3-siRNA). G-Rg3 group cells were given G-Rg3 (40 mg/L) intervention for 48 h; 24h after G-Rg3+DKK3-NC group cells were transfected with DKK3-NC, G-Rg3 intervention was conducted 48 h; after G-Rg3+DKK3-siRNA group cells were transfected, G-Rg3 intervention was conducted 48 h; cells in the control group were cultured normally. MTT method was used to detect cell proliferation; flow cytometry was used to detect cell apoptosis; Western blotting was used to detect the expression of DKK3, Wnt, and β-catenin. Results: Compared with the control group, the apoptosis index of G-Rg3 group was significantly increased (P<0.01), cell proliferation was significantly decreased (P<0.01), DKK3 expression was significantly increased (P<0.01), Wnt and β-catenin expression were significantly decreased (P<0.01); compared with the G-Rg3 group, the apoptosis index in the G-Rg3+DKK3-siRNA group was significantly reduced (P<0.01), cell proliferation was significantly increased (P<0.01), DKK3 expression significantly decreased (P<0.01), and the expression of Wnt and β-catenin increased significantly (P<0.05 or P<0.01). Conclusion: G-Rg3 can inhibit the proliferation of liver cancer cells and promote apoptosis. The mechanism is related to the promotion of DKK3 expression and inhibition of Wnt/β-catenin signal transduction.

Key words： Liver cancer Ginsenoside Rg3 Dickkopf-related protein 3 Apoptosis Proliferation

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	Articles by authors
	HU Shanshan
	WANG Shaowen
	JIANG Lei

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HU Shanshan,WANG Shaowen,JIANG Lei. The Effect of Ginsenoside Rg3 Regulateing Dickkopf-Related Protein 3 on the Proliferation and Apoptosis of Hepatocellular Carcinoma Cells[J]. HeBei Med, 2022, 28(1): 13-16.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2022.01.03 OR http://www.hbyxzzs.cn/EN/Y2022/V28/I1/13

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