|
|
|
| Clinical Significance the Changes of Serum Adropin and ZO-1 Levels in Patients with Hypertensive Cerebral Hemorrhage |
| ZHANG Bin, MENG Wei, GAO Weijun, et al |
| Panjin Central Hospital, Liaoning Panjin 124010, China |
|
|
|
|
Abstract Objective: To explore the expression levels of Adropin and zonula occludens-1 (ZO-1) in patients with hypertensive cerebral hemorrhage and its clinical significance. Methods: A total of 143 patients with hypertensive cerebral hemorrhage in our hospital from March 2017 to March 2020 were collected and divided into mild group (n=69, NIHSS score:1~14), moderate group (n=43, NIHSS score:15~20), and severe group (n=31, NIHSS score: 21-42) according to the degree of neurological deficit. The patients also were divided into good prognosis group (n=107, GOS score: 4~5) and poor prognosis group (n=36, GOS score: 1~3) according to the prognosis 6 months after treatment. At the same time, 71 healthy volunteers were randomly selected as control group. Serum Adropin and ZO-1 levels were detected by enzyme-linked immunosorbent assay. The receiver operating characteristic (ROC) curve was plotted to evaluate the predictive value of serum Adropin and ZO-1 for poor prognosis. Results: The serum Adropin level in case group was lower than that in control group [(1.53±0.41)μg/ml vs. (3.79±0.46)μg/ml; t=36.449], and ZO-1 level was higher than that in control group [(308.22±60.93)pg/ml vs. (149.86±51.04)pg/ml],the difference was statistically significant (P<0.05). The serum Adropin in severe group and moderate group was lower than that in mild group, and ZO-1 level was higher than that in mild group. Moreover, the serum Adropin in severe group was lower than that in moderate group, and ZO-1 level was higher than that in moderate group, the difference was statistically significant (P<0.05). The Adropin level in poor prognosis group was lower than that in good prognosis group, and ZO-1 level was higher than that in good prognosis group, the difference was statistically significant (P<0.05) . Pearson product moment correlation analysis showed that serum Adropin level was negatively associated with NIHSS score (r=-0.792, P<0.05), and positively associated with GOS score (r=0761, P<0.05). Serum ZO-1 level was positively associated with NIHSS score (r=0.708, P<0.05), and negatively associated with GOS score (r=-0.673, P<0.05). The result of ROC curve showed that serum Adropin and ZO-1 had predictive value for the poor prognosis of patients with hypertensive cerebral hemorrhage 6 months after treatment, the AUC was 0.790 and 0.761 respectively, and the Adropin combined with XO-1 had higher predictive value, the AUC was 0.837, the sensitivity and specificity were 83.04% and 78.65% respectively. The predictive value of combined detection was better than that of single index (P<0.05). Conclusion: The down-regulation of serum Adropin expression and up-regualtion of ZO-1 expression are involved in the process of hypertensive cerebral hemorrhage, which are closely related to the degree of neurological deficit and prognosis. Early detection of Adropin and ZO-1 can be used as important biochemical index to judge the severity of the disease and predict the prognosis.
|
|
|
|
|
|
[1] 王旭生,周祥,郭言,等.急性高血压性脑出血术后患者抑郁及其危险因素分析[J].中国慢性病预防与控制,2020,28(7):513~517.
[2] Mushala BAS,Scott I.Adropin:a hepatokine modulator of vascular function and cardiac fuel metabolism[J].Am Physiol Heart Circ Physiol,2021,320(1):238~244.
[3] 刘俊霞,白成芝,杨林.Adropin蛋白对急性心肌梗死患者预后的评估价值[J].解放军预防医学杂志,2019,37(7):7~8.
[4] Mohammadi MT.Overproduction of nitric oxide intensifies brain infarction and cerebrovascular damage through reduction of claudin-5 and ZO-1 expression in striatum of ischemic brain[J].Pathol Res Pract,2016,212(11):959~964.
[5] 中华医学会.临床诊疗指南.神经外科学分册2012版[M].北京:人民卫生出版社,2013.77~79.
[6] Runde D.Calculated Decisions:NIH stroke scale/score (NIHSS)[J].Emerg Med Pract,2020,22(7):6~7.
[7] Reith FC,Synnot A,van den Brande R,et al.Factors influencing the reliability of the glasgow coma scale:a systematic review[J].Neurosurgery,2017,80(6):829~839.
[8] 宋福林,胡欢,李萍.Adropin蛋白在心脑血管疾病中的作用[J].生命的化学,2021,41(3):501~505.
[9] 王金花,孔祥勇,姚勇.血清adropin水平与心房颤动患者心房重构的相关性研究[J].心脑血管病防治,2020,20(5):467~470.
[10] Wang H,Li T,Zhao L,et al.Dynamic effects of Ioversol on the permeability of the blood-brain barrier and the expression of ZO-1/Occludin in rats[J].Mol Neurosci,2019,68(2):295~303.
[11] 王蔚,钟霞,杜渊,等.蛭龙活血通瘀胶囊对模型大鼠脑出血后脑组织水肿及ZO-1、Occludin的影响[J].中国中医急症,2020,29(12):2084~2086,2096. |
|
|
|
2021, Vol. 27 
