NSUN2在口腔鳞癌中表达上调且通过NF-κB通路调控癌细胞发展

doi:10.3969/j.issn.1006-6233.2021.10.004

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Abstract Objective: To investigate the effect of NSUN2 on proliferation, invasion and EMT of oral squamous cell carcinoma and its mechanism. Methods: The gene levels of NSUN2 in the tissues of oral squamous cell carcinoma patients, paracancerous tissues, oral squamous cell carcinoma OSCC cell lines(SCC9 and CAL27 cells)and human normal oral epithelial keratinocytes(HOK cells)were detected by qRT-PCR. The siRNA NC, NSUN2 siRNA1 and NSUN2 siRNA2 plasmids were transfected into CAL27 cells, respectively. The proliferation, invasion and EMT ability of CAL27 cells were detected by MTT, cell invasion assay and Western blot, respectively. After transfected with pcDNA-3.1(+) and pcDNA-NSUN2 plasmid, the effects of up-regulation of NSUN2 on proliferation, invasion and EMT of CAL27 cells were detected. The effect of down-regulation or overexpression of NSUN2 on the expression of NF-κB pathway related proteins was detected by Western blot. NF-κB pathway inhibitor SN50 was used to detect the effect of up-regulation of NSUN2 on the development of CAL27 cells. Results: Compared with paracancerous tissues, the expression of NSUN2 in oral squamous cell carcinoma tissues was significantly up-regulated(P<0.01).Compared with HOK cells, NSUN2 expressions in SCC9 and CAL27 cells were significantly up-regulated(P<0.01).The proliferation, invasion and EMT abilities of CAL27 cells transfected with NSUN2 siRNA1 and NSUN2 siRNA2 were significantly lower than those of the siRNA NC group, and the ratio of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly decreased(P<0.01);The proliferation, invasion and EMT abilities of CAL27 cells in pcDNA-NSUN2 transfected group were significantly higher than those in pcDNA-3.1(+) group, and the ratios of p-TAK1/TAK1 and NF-κB p-p65/NF-κB p65 were significantly increased(P<0.01). After treated with SN50, the promoting effect of NSUN2 on the development of CAL27 cells was significantly down-regulated. Conclusion: NSUN2 was up-regulated in oral squamous cell carcinoma cells and promoted the proliferation, invasion and EMT of CAL27 cells through NF-κB pathway.

Key words： NSUN2 NF-κB Oral squamous cell carcinoma Proliferation

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	GAO Duoduo
	WANG Yao

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GAO Duoduo,WANG Yao. Overexpression of NSUN2 Promoted Proliferation and Metastasis of Cervical Cancer Cells Through MiR-27b/FZD7 Axis[J]. HeBei Med, 2021, 27(10): 1603-1607.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.10.004 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I10/1603

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