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| Comparison of Fluorescence in Situ Hybridization Detection of HER-2 Gene Amplification and Immunohistochemical Detection of C-erbB-2 Protein Expression |
| CHEN Xu, et al |
| The People's Hospital of Baise, Guangxi Baise 533000, China |
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Abstract Objective: To compare the differences and correlation between fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) in the detection of HER-2 gene and protein in breast cancer. Methods: Taking the surgical pathological slices of 98 breast cancer patients as the test objects, FISH and IHC were performed respectively, and the test results were analyzed. Results: IHC test results showed that 53 cases (54.08%) were positive and 45 cases (45.92%) were negative. FISH test results showed that 29 cases (29.59%) were positive and 69 cases (70.41%) were negative. With FISH as the gold standard, the coincidence rate of negative (-) for IHC is 42 (93.33%), the coincidence rate of positive (+) is 5 (17.86%), the coincidence rate of ++ is 11 (78.57%), and the coincidence rate of +++ is 10 (90.91) %). Conclusion: In IHC preliminary screening of C-erbB-2 protein, the coincidence rate of -, +++ is relatively high, and the coincidence rate of IHC in the screening of ++ and + is low. It is recommended to do FISH screening on the basis of IHC screening to clarify the HER-2 gene status.
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2021, Vol. 27 
