养心定悸胶囊在缺血再灌注损伤心肌组织中的保护作用研究

doi:10.3969/j.issn.1006-6233.2021.07.029

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Abstract Objective: To study the effect of Yangxindingji Capsule on nitrogen metabolism in myocardial tissue after ischemia-reperfusion injury. Methods: A model of rats with ischemia-reperfusion injury was established. The activities of LDH and CK in the myocardial tissue of the model rats were investigated. The content of antioxidant enzymes and lipid peroxidation products, the content of humic, spermine and polyamine in the tissues, Ornithine decarboxylase (ODC), spermine / spermine N-acetyltransferase activity (SSAT), nitric oxide (no) and nitrogen free radical content (ONOO-) were investigated. The mitochondrial related metabolic enzyme activity of myocardial tissue was detected. The effect of Yangxindingji capsule on the prognosis was evaluated. Results: The activities of LDH and CK isoenzyme in the myocardial tissue of the model rats were significantly increased, and the myocardial damage was significant. The content of polyamine, humic acid and spermine in the myocardial tissue of the model rats was significantly increased, the activity of ODC and SSAT increased, the NO content was significantly decreased, the activity of ONOO- of nitrogen free radical was significantly increased, the activity of superoxide dismutase, catalase and glutathione peroxidase in the myocardial tissue was significantly decreased, and the content of thiobarbituric acid reactant and lipid peroxide increased significantly, The oxidative stress response of myocardial tissue was significant. The mitochondrial metabolic enzymes isocitrate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, The activity of α-ketoglutarate dehydrogenase was significantly reduced, which indicating that the energy metabolism of mitochondria was abnormal. The results showed that the Yangxindingji capsule could significantly improve the myocardial injury, reduce the LDH and CK activity of myocardial injury index, and give yangxindingji capsule to dry prognosis, significantly reduce the activity level of ODC and SSAT, reduce the content of polyamine, humic, spermine, nitrogen free radical, restore the level of NO content, improved the metabolism of myocardial nitrogen, and restored the enzyme activity related to antioxidant damage in heart tissue, The content of thiobarbituric acid reactant and lipid peroxide was reduced, and the activity of mitochondrial metabolizing enzyme was restored.Conclusion: Yangxindingji capsule could significantly improve the abnormal nitrogen metabolism of myocardial tissue, reduce the content of amine, reduce nitrogen free radicals, restore the energy metabolism of myocardial tissue, and reduce the occurrence of myocardial tissue injury.

Key words： Yangxindingji capsule Ischemia reperfusion injury Nitrogen metabolism Oxidative stress injury

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.07.029 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I7/1191

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