黄芪注射液联合阿托伐他汀对急性肺损伤大鼠肺纤维化及细胞黏附功能的影响

doi:10.3969/j.issn.1006-6233.2021.05.04

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Abstract Objective: To study the effects of astragalus injection combined with atorvastatin on pulmonary fibrosis and cell adhesion function in rats with acute lung injury.Methods: 60 wistar rats were randomly divided into sham operation group (control group),model group,atorvastatin group (statin group),Astragalus group and astragalus group combined atorvastatin group (combination group),with 12 rats in each group.The pulmonary fibrosis model of acute lung injury was established.After the beginning of the experiment,the Astragalus group and the combined group were intraperitoneally injected with 1.0ml·kg^-1·d^-1 astragalus injection every day,and the statin group and the combined group were given atorvastatin 20 mL ·kg^-1·d^-1 gavage.At the same time,the control group,model group and statin group were given intraperitoneal injection of the same amount of normal saline.The control group,model group and Astragalus group were given the same amount of normal saline.The rats in each group were sacrificed 6h after administration on day 14 for histopathological observation and scoring,and the expression changes of Hydroxyproline (HyP),tumor necrosis factor (TNF-α),intercellular adhesion molecule (ICAM-1),vascular intercellular adhesion molecule (VCAM-1) and platelet endothelial adhesion molecule (PECAM-1) in lung tissues of each group were detected.Results: Compared with the control group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 expression,alveolitis and pulmonary fibrosis scores of the other four groups were significantly enhanced (P<0.05).Compared with the model group,HyP,TNF-α,ICAM-1,VCAM-1 and PECAM-1 in the lung tissues of the statins group,the Astragalus group and the combination group were significantly lower (P<0.05),and the roots of the combination group were significant.Conclusion: The expressions of ICAM-1,VCAM-1,TNF-α,PECAM-1 and HyP in the pulmonary fibrosis tissues with acute lung injury were significantly unbalanced,while the astragalus,statins and the combined group were able to significantly correct their imbalances,reduce alveolitis and pulmonary fibrosis scores,and thus effectively slow down the occurrence and development of pulmonary fibrosis with acute lung injury.Moreover,the combined group was more effective..

Key words： Acute lung injury pulmonary fibrosis Astragalus Atorvastatin Adhesive molecule TNF-α Hydroxyproline

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.05.04 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I5/719

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