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| Expression and Clinical Significance of Muscarinic Acetylcholine Receptor M3 in Hilar Cholangiocarcinoma |
| ZHANG Hongwei, WANG Cairu, MIAO Guodong, et al |
| Chengde Central Hospital, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the expression of muscarinic acetylcholine receptor m3 (CHRM3) in hilar cholangiocarcinoma and its relationship with prognosis.Methods: A total of 69 cases of hilar cholangiocarcinoma and 69 cases of normal cholangiocarcinoma adjacent to cholangiocarcinoma resected by surgery in our hospital from January 2013 to April 2017 were selected as research objects. The expression of CHRM3 in hilar cholangiocarcinoma and paracancerous tissues was detected by immunohistochemistry, the relationship between the expression level of CHRM3 and the clinicopathological features of hilar cholangiocarcinoma were analyzed, and the relationship between the expression of CHRM3 and the prognosis of hilar cholangiocarcinoma was explored after follow-up for 3 years. Results: The positive rate of CHRM3 in the adjacent tissues and hilar cholangiocarcinoma was 7.25% and 84.06%, respectively; the difference was statistically significant (P<0.05). The expression level of CHRM3 in hilar cholangiocarcinoma was not related to age and gender (P>0.05), but related to pathological stage, pathological grade, lymph node metastasis and nerve infiltration (P<0.05). Kaplan-Meier method showed that the mean survival time of patients with CHRM3 negative hilar cholangiocarcinoma was (33.69 ± 0.76) months, which was significantly longer than (23.37 ± 1.02) months of CHRM3 positive patients (P<0.05). Multivariate analysis showed that nerve infiltration and positive CHRM3 were independent risk factors for poor prognosis in patients with hilar cholangiocarcinoma (P<0.05). Conclusions: The positive expression rate of CHRM3 in hilar cholangiocarcinoma is significantly increased, which is related to pathological stage, pathological grade, lymph node metastasis and nerve infiltration. The increased expression rate of CHRM3 is an independent risk factor for poor prognosis in hilar cholangiocarcinoma.
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2021, Vol. 27 
