重组慢病毒介导STAT3过表达质粒转染促进显性脊柱裂胎鼠骨髓间充质干细胞向神经细胞分化

doi:10.3969/j.issn.1006-6233.2021.03.007

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Abstract Objective: To observe the action of recombinant lentivirus mediated STAT3 overexpression plasmid transfected into bone marrow mesenchymal stem cells on neural cell trans-differentiation in spinal cord tissue of fetal rats with spina bifida aperta. Methods: The fetal rat model of spina bifida aperta was established by retinoic acid and treated by microsurgical injection of stem cells. They were divided into blank control group (12 cases),negative control group (10 cases) and experimental group (11 cases). OD value of BMSCs viability was determined by CCK-8 kit. The expression of STAT3,pSTAT3,nerve markers (GFAP,NSE,NF and nestin) and apoptosis related factors (Caspase-8 and Bcl-2) were tested by the method of real-time PCR and Western blot. Results: OD value in experimental group was the highest at 8 hours after transplantation by CCK-8 kit,and then gradually decreased,which was statistically significant compared with blank control group and negative control group (P<0.05). There was no statistical difference in OD peak time and value between blank control group and negative control group. The expression levels of pSTAT3,GFAP,NSE,NF and nestin in experimental group were remarkably higher than those in blank control group and negative control group (P<0.05),but STAT3 expression in experimental group were statistically decreased (P<0.05). The relative expression levels of Caspase-8 and Bcl-2 in experimental group were tremendously lower than those in blank control group and negative control group (P<0.05). Conclusion: Transfection of recombinant lentivirus mediated STAT3 overexpression plasmid with BMSCs can improve the efficiency of transforming into neural cells and provide new seed cells for the treatment of congenital spina bifida aperta.

Key words： Bone marrow mesenchymal stem cells Spina bifida aperta Signal transducer and activator of transcription 3 Lentivirus transfection

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.03.007 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I3/384

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