CDKN3和FOXA1 mRNA在直肠癌中的表达关系及意义

doi:10.3969/j.issn.1006-6233.2021.01.019

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Abstract Objective: To investigate the expressions and clinical significance of cyclin dependent kinase inhibitor 3 (CDKN3) and forked head box A1 (FOXA1) mRNA in rectal cancer. Methods: 94 rectal cancer patients in Tangshan Union Hospital and the 82nd Army Hospital of the PLA from November 2017 to March 2020 were used as the observation group, and 95 healthy people who had routine physical examination in our hospital in the same period as the control group; the expressions of CDKN3 and FOXA1 mRNA in serum of patients with rectal cancer were detected by real-time fluorescent quantitative PCR (QRT PCR); the expression levels of CDKN3 and FOXA1 mRNA in serum of patients with high and low grade rectal cancer were analyzed; the relationships between the levels of CDKN3 and FOXA1 mRNA and the clinicopathological characteristics of rectal cancer patients were analyzed; and the diagnostic values of CDKN3 and FOXA1 mRNA levels in patients with high and low rectal cancer were predicted. Results: The expression levels of CDKN3 and FOXA1 mRNA in the observation group were significantly higher than those in the control group (P<0.05); the expressions of CDKN3 and FOXA1 mRNA in the serum of patients with high rectal cancer were significantly higher than those of patients with low rectal cancer (P<0.05); in the serum of patients with rectal cancer the expression levels of CDKN3 and FOXA1 mRNA were related to TNM stage, differentiation degree and lymph node metastasis (P<0.05); the area under ROC curve of CDKN3 mRNA in the diagnosis of high and low grade rectal cancer was 0.839, the cutoff value was 5.317, the sensitivity was 63.8%, and the specificity was 94.4%; the area under ROC curve of FOXA1 mRNA in the diagnosis of high and low grade rectal cancer was 0.853 , the cutoff value was 0.740, the sensitivity was 70.7%, and the specificity was 97.2%. Conclusions: The expressions of CDKN3 and FOXA1 mRNA in serum of patients with rectal cancer are increased, in addition, the expressions of them in patients with high-grade rectal cancer are significantly higher than those in patients with low-grade rectal cancer, CDKN3 and FOXA1 mRNA are involved in the process and development of rectal cancer, and have certain values in the diagnosis of high and low grade rectal cancer.

Key words： Rectal cancer Cyclin-dependent kinase inhibitor 3 Fork head box protein A1 Expression significance

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.01.019 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I1/84

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