MicroRNA-193a 通过靶向 WT1抑制肝细胞癌发生的机制研究

doi:10.3969/j.issn.1006-6233.2021.01.001

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Abstract Objective: To elucidate the function of miR-193a in HCC. Methods: The expression levels of miR-193a and Wilms' tumor-1 (WT1) were detected by quantitative real Time-polymerase chain Reaction (qRT-PCR) assay. In addition, miR-193a expression was also investigated in MHCC97H,HCCLM3,Huh7 and LO₂ cell lines. Moreover, the HCC proliferation was examined through cell counting Kit-8 (CCK-8) assay after miR-193a overexpression or inhibition. Tumor growth of HCC was observed via xenograft tumor formation assay. The relationship between miR-193a and WT1 was verified by the dual-Luciferase assay. Results: Downregulation of miR-193a whereas upregulation of WT1 were identified in HCC, which predicted a worse prognosis in HCC patients. Meanwhile, miR-193a was significantly decreased in LO₂ cell lines compared with MHCC97H,HCCLM3,Huh7 cell lines. Low miR-193a expression HCC patients showed severer TMN stage and presence of lymph node metastasis. Furthermore, it was found that miR-193a mimic suppressed cell proliferation by inhibiting WT1. miR-193a also inhibited tumor growth of HCC. Conclusion: miR-193a suppressed tumorigenesis of HCC by targeting WT1.

Key words： Hepatocellular carcinoma MicroRNA-193a WT1

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	LIU Yaqi
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LIU Yaqi,et al. Mechanism of MicroRNA-193a Suppressing the Tumorigenesis of Hepatocellular Carcinoma Through Targeting WT1[J]. HeBei Med, 2021, 27(1): 1-4.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2021.01.001 OR http://www.hbyxzzs.cn/EN/Y2021/V27/I1/1

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