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| Clinical Significance and Expression of Cx43 and p70S6K in Gastric Cancer |
| LIU Shasha , LIU Huan , ZHAO Yang , et al |
| The Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China |
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Abstract Objective: To investigate the expression of connexin 43(Cx43) and p70S6 kinase (p70S6K) in gastric cancer tissues and their correlation with the clinicopathological parameters.Methods: The protein expression of Cx43 and p70S6K in gastric cancer and normal gastric mucosa was detected by Western blot assay.The mRNA expression of Cx43 and p70S6K genes was detected by quantitative real-time PCR.Results: At the protein level,the expression of Cx43 in gastric cancer tissues M(0.39,0.98) was lower than that in normal gastric mucosa tissues M(1.62,3.80),and the expression level of p70S6K M(0.94,1.55) was higher than that in normal gastric mucosa tissues M(0.54,1.02),and the differences were statistically significant (all P<0.01).At the gene level,the positive expression of Cx43 mRNA in gastric cancer tissues M(0.33,0.66)was lower than that in normal gastric mucosa tissues M(1.00,1.00),and the positive expression of p70S6K mRNA in gastric cancer tissues M(2.14,4.14) was significantly higher than that in normal gastric mucosa tissues M(1.00,1.00),and the differences were statistically significant (all P<0.01).No matter at the protein level or gene level,with the worse differentiation of gastric cancer,the higher the TNM stage,the deeper the infiltrating muscle layer,and the lower the positive expression rate of Cx43 in lymph node metastasis;on the contrary,the positive expression rate of p70S6K increased significantly (all P<0.05),regardless of gender and age (all P>0.05).There was a negative correlation between Cx43 and p70S6K protein expression in gastric cancer tissues (r=-0.746,P＜0.01).There was a negative correlation between Cx43 and p70S6K mRNA expression (r=-0.759,P＜0.01).Conclusion: The abnormal expression of Cx43 and p70S6K in gastric cancer may play an important role in the occurrence and development of gastric cancer.
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2020, Vol. 26 
