miR-335-5p和转录因子ATF2在宫颈癌中的表达关系研究

doi:10.3969/j.issn.1006-6233.2020.10.08

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Abstract Objective: To investigate the expressions of microRNA-335-5p (miR-335-5p) and activated transcription factor 2 (ATF2) in cervical cancer and their relationship with clinicopathological characteristics of cervical cancer patients. Methods: 72 patients with cervical cancer who underwent surgical treatment in our hospital from August 2017 to October 2019 were selected as the experimental group, 65 patients with uterine leiomyoma who underwent surgical treatment in the same period were selected as the control group, and cervical tissues of cervical cancer and uterine leiomyoma patients who underwent surgical resection were taken as the research samples. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression of miR-335-5p, ATF2 mRNA and matrix metalloproteinase 2(MMP2) mRNA; immunohistochemistry was used to detect the positive expression rate of ATF2 and MMP2 protein; the relationships between the expression of miR-335-5p, ATF2 and the clinicopathological characteristics of cervical cancer were analyzed; the factors affecting cervical cancer were analyzed by logistic regression analysis; and the correlation of expression of mir-335-5p, Atf2 and MMP2 was analyzed. Results: The expression levels of miR-335-5p, ATF2 mRNA and MMP2 mRNA in the experimental group were significantly higher than those in the control group (P<0.05). The positive expression rate of ATF2 and MMP2 protein in the experimental group was significantly higher than that in the control group (P<0.05). The expression levels of miR-335-5p, ATF2 and MMP2 in cervical cancer tissues were correlated with FIGO stage, depth of invasion and lymph node metastasis (P<0.05). Logistic multivariate regression analysis showed that miR-335-5p, ATF2 and MMP2 were risk factors for cervical cancer. There was a positive correlation between the expression of miR-335-5p, Atf2 and MMP2 in cervical cancer (P<0.05). Conclusion: The expressions of miR-335-5p and ATF2 are high in cervical cancer tissues. Both of them are related to FIGO stage, depth of invasion and lymph node metastasis, and they may be biological indicators affecting the occurrence and development of cervical cancer.

Key words： MicroRNA-335-5p Activated transcription factor 2 Cervical cancer

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	RAN Wei
	LIAO Ping
	LIU Xinglan
	et al

Cite this article:

RAN Wei,LIAO Ping,LIU Xinglan, et al. The Relevance Between the Expressions of MiR-335-5p and Transcription Factor ATF2 in Cervical Cancer[J]. HeBei Med, 2020, 26(10): 1617-1623.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.10.08 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I10/1617

[1] 丁波,孙玮,沈杨,等.TNF-α基因rs1800629多态性与中国人群宫颈癌的易感性[J].中华疾病控制杂志,2019,23(7):850~855.
[2] Abudula A, Rouzi N, Xu L, et al. Tissue-based metabolomics reveals potential biomarkers for cervical carcinoma and HPV infection[J]. Bosnian Basic Med, 2020,20(1):78~87.
[3] Sonohara F, Yamada S, Takeda S, et al. Exploration of exosomal micro RNA biomarkers related to epithelial-to-mesenchymal transition in pancreatic cancer[J]. Anticancer Res, 2020,40(4):1843~1853.
[4] Dong Y, Liu Y, Jiang A, et al. MicroRNA-335 suppresses the proliferation, migration, and invasion of breast cancer cells by targeting EphA4[J]. Mol Cell Biochem, 2018, 439(1~2):95~104.
[5] Liu J, Bian T, Feng J, et al. miR-335 inhibited cell proliferation of lung cancer cells by target Tra2β[J]. Cancer Sci, 2018, 109(2):289~296.
[6] Huebner K, Procházka J, Monteiro AC, et al. The activating transcription factor 2: an influencer of cancer progression[J]. Mutagenesis, 2019,34(5~6):375~389.
[7] 陈璐彦,孙耀,许朱镕,等.Notch4通过调控活化转录因子2影响胰腺癌细胞的迁移和侵袭及其可能的机制[J].中国肿瘤生物治疗杂志,2020,27(2):156~160.
[8] Zhang J, Li D, Zhang Y, et al. Integrative analysis of mRNA and miRNA expression profiles reveals seven potential diagnostic biomarkers for non-small cell lung cancer[J]. Oncol Rep, 2020,43(1):99~112.
[9] Jia Q, Ye L, Xu S, et al. Circular RNA 0007255 regulates the progression of breast cancer through miR-335-5p/SIχ² axis[J]. Thorac Cancer, 2020,11(3):619~630.
[10] Liu R, Guo H, Lu S, et al. MiR-335-5p restores cisplatin sensitivity in ovarian cancer cells through targeting BCL2L2[J]. Cancer Med, 2018,7(9):4598~4609.
[11] 吕晓峰,洪汉卿,崔世红,等.微小RNA-451表达对卵巢癌细胞增殖能力及凋亡水平的影响[J].中国临床药理学杂志,2018,34(9):1095~1099.
[12] Voloshanenko O, Schwartz U, Kranz D, et al. β-catenin-independent regulation of Wnt target genes by RoR2 and ATF2/ATF4 in colon cancer cells[J]. Sci Rep, 2018, 8(1):3178~3190.
[13] Geng L, Chen X, Zhang M, et al. Ubiquitin-specific protease 14 promotes prostate cancer progression through deubiquitinating the transcriptional factor ATF2[J]. Biochem Bioph Res Co, 2020,524(1):16~21.