|
|
|
| Comparative Experimental Study on Three Different Materials for Repairing Oral Mucosa Defects in Rats |
| HAN Yongjie, LIU Shijie, DENG Lu |
| Qinghai Provincial People's Hospital, Qinghai Xining 810007, China |
|
|
|
|
Abstract Objective: To investigate the effects of three kinds of materials, namely, homologous (human skin) acellular matrix, heterogeneous (cowhide) acellular matrix, and nanocellulose protein in the repair of oral mucosa defects in rats. Methods: 80 3-month-old male Wister clean rats were randomly divided into three groups: group A (nanocellulose silk fibroin group, n=20), group B (cowhide acellular matrix group, n=20), group C (human skin acellular matrix group, n=20), and group D (blank control group, n=20). Full-thickness buccal mucosa with a diameter of 10 mm was excised from rats in each group. Wounds of rats in groups A, B, and C were respectively covered with silk fibroin, heterogeneous (cowhide) acellular matrix, and homologous (human skin) acellular matrix which had the same diameter and were treated with radicidation. The wounds of rats in group D were covered with vaseline gauze. The wound areas were compared among groups at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery. The numbers of fibroblasts, neovascular endothelial cells and inflammatory cells, and growth of endothelial cells in rats at different time points were analyzed through HE staining and immunohistochemistry. Results: ①There was no wound infection in each group. Transparent membranes could be observed in the grafted area of group A, B, and C at 1 week after surgery. The wound margin of group D was slightly red and swollen. The wound diameter of group D was significantly larger than that of group A, B, and C at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference in wound size among group A, B, and C at each time point (P>0.05). ② HE staining showed that the numbers of fibroblasts and inflammatory cells in group A, B, and C at different time points were significantly smaller than those in group D (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). ③Immunohistochemistry showed that the expression of CK-positive cells in group A, B, and C was significantly higher than that in group D at 1 week, 2 weeks, 3 weeks and 4 weeks after surgery (P<0.05). The number of CD34-positive cells was significantly larger than that in group D at 1 week, 2 weeks, and 3 weeks after surgery (P<0.05), without significant difference among group A, B, and C at each time point (P>0.05). Conclusion: The effects of nanocellulose protein, autologous and allogeneic cell matrices are similar in repair of oral mucosa damage in rats.
|
|
|
|
|
|
[1] Hiroaki Tsuno,Motonori Okabe,Kumiko Fujiwara,et al.Clinical application and evaluation of hyperdried human amniotic membrane for the surgical repair of oral mucosal defects[J].Oral Science International,2019,16(11):55~58.
[2] 李建成,宋培军,杨东坤,等.游离小腿后外侧穿支皮瓣在口腔颌面部组织缺损修复重建中的应用[J].中华显微外科杂志,2017,40(3):248~251.
[3] H Shao,M Sun,F Zhang,et al.Custom repair of mandibular bone defects with 3D printed bioceramic scaffolds[J].Journal of Dental Research,2017,97(1):002203451773484.
[4] Fernanda Gonalves Basso,Taisa N Pansani,Diana G Soares,et al.Influence of bisphosphonates on the adherence and metabolism of epithelial cells and gingival fibroblasts to titanium surfaces[J].Clin Oral Investig,2017,22(2):1~8.
[5] Wang QX,Qi XY,Dong ZM,et al.Effect of bone marrow mesenchymal stem cells on hydroxyapatite/silk fibroin/chitosan composite 3D scaffold for rat skull defects repair[J].Journal of Biomaterials&Tissue Engineering,2017,8(10):15~17.
[6] 王忠朝,范丽苑,蔡炜,等.纳米纤维丝素蛋白和脱细胞基质修复口腔黏膜[J].中国组织工程研究,2016,(21):3104~3109.
[7] Shi WL,Sun MY,Hu XQ,et al.Structurally and functionally optimized silk-fibroin-gelatin scaffold using 3D printing to repair cartilage injury in vitro and in vivo[J].Advanced Materials,2017,29(29):52~54. |
|
|
|
2020, Vol. 26 
