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| Clinical Comparison of Two Technology Assay Results in Detection of the Blood Type Antibody IgG Subsets |
| MA Yintu, et al |
| 980 Hospital of PLA Joint Logistics Support Forces, Hebei Shijiazhuang 050082, China |
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Abstract Objective: Apply the micro column gel technique assay ( MGTA) and the ELISA in detection the blood type antibody IgG subsets,evaluate the application effect to screening the antibody IgG subsets and the relationship between the antibody IgG subsets and HDN.Methods: By means of microcolum gel test and ELISA,IgG subsets were analyzed in 62 pregnant women who had the IgG anti-A(B) titer higher than 512 in 57 cases O maternal serum and the IgG anti-D titer higher than 64 in 5 cases Rh negative maternal serum.The 27 newborn cases were screened the IgG subsets and the three tests. The consistency and relativity of two methods were compared. Results:The consistency of two tests was well. The results of 5 cases Rh and 44 in 57 ABO cases were accorded.The results higher of MGTA were 6 cases and lower were 7 cases compared to ELISA, while there was no significant difference between MGTA and ELISA(P>0.05).The content of antibody IgG subsets in newborn was closely related with the mather.The IgG1 and IgG3 in postnatal haemolytic newborns were higher distinctly and the more content high the more jaundice early and serious.Conclusion: The two technology all should be used in screening the IgG antibody subsets. As for a general clinical application,the MGTA was more advantaged than ELISA because of higher accuracy and easier procedure in screening the contents of IgG antibody subsets. The method may provide a directional significance in early intervention of blood group incompatibility hemolytic disease of the newborns.
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2020, Vol. 26 
