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| Diagnostic Value of Serum MicroRNA 122 and Microrna-130a Levels in Occult Hepatitis B Virus Infection |
| XU Lanfeng, WANG Qian, LIU Hongling, et al |
| Jinzhou Central Hospital, Liaoning Jinzhou 121001, China |
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Abstract Objective: To study the correlation between serum microRNA-122 (miR-122) and miR-130a and occult hepatitis B (OBI), and to explore their diagnostic value for OBI. Methods: 60 patients with OBI (OBI group), 60 carriers of inactive HBsAg (ASC) (ASC group), 60 patients with chronic hepatitis B (CHB) (CHB group) and 60 suspicious patients (control group) were selected. The expression of serum miR-122 and miR-130a was detected by real-time fluorescence quantitative method. Results: The relative expression level of serum miR-122 in OBI group was significantly lower than that in the other three groups (all P<0.05). However, the relative expression level of miR-130a was significantly higher than that in the three groups (all P<0.05). The relative expression level of serum miR-122 in OBI group, the control group, ASC group and CHB group increased in turn (all P<0.05), and the relative expression level of miR-130a in OBI group, CHB group, ASC group and control group decreased in turn (all P<0.05). The value of miR-130a/miR-122 in OBI group was significantly higher than that in the other three groups (all P<0.05). Pearson correlation analysis showed that the expression of serum miR-122 was negatively correlated with the expression of miR-130a in patients with OBI (r=0.421, P<0.05). ROC curve analysis showed that the areas under the curves of serum miR-122, miR-130a and miR-130a/miR-122 value for differentiating patients with OBI and suspicious patients were 0.859 (95% CI: 0.762~0.955), 0.844 (95% CI: 0.739~0.950) and 0.945 (95% CI: 0.018~0.989), respectively. The areas under the curves for differentiating OBI and ASC were 0.942 (95% CI: 0.021~0.995), 0.813 (95% CI: 0.699~0.927) and 0.964 (95% CI: 0.012~0.997), respectively. The areas under the curves for differentiating OBI and CHB were 0.998 (95% CI: 0.000~1.000), 0.646 (95% CI: 0.503~0.789) and 0.993 (95% CI: 0.000~1.000), respectively. Conclusion: The decreased expression of serum miR-122 and the increased expression of miR-130a are associated with OBI, which is of important diagnostic value for OBI. The diagnostic value of miR-130a/miR-122 is higher than that of serum miR-122 and miR-130a alone.
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2020, Vol. 26 
