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| Effect of Shenshao Oral Liquid on CD14 / TLR4 Mediated Secondary Inflammatory Injury in the Brain of Rats with Experimental Cerebral Hemorrhage |
| LIU Hongwei, TANG Xingguo, LI Binbin, et al |
| Chengde Nursing Vocational College, Hebei Chengde 067000, China |
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Abstract Objective: To research Shenshao Oral Liquid Inhibits Secondary Inflammatory Injure Induced by CD14/TLR4 after Intracerebral Hemorrhage in Experimental Rats. Methods: Experimental rats were randomly divided into sham operation group, model group and intervention group. Each group further was subdivided into 6 h group, 12 h group, 24 h group, 72 h group, and 7 d group. ICH group and SS group were used to duplicate the model of cerebral hemorrhage. SS group was given Shenshao oral liquid (8ml/d) by gavage after waking up. The number and protein expression of CD14, TLR4 and IL-8 positive cells were detected by immunohistochemistry and Western blotting. Results: The edema was appeared after 6h after the ICH model was established, and the water content of brain was gradually increased at 12h to 72h, and reached to the highest level at 72h, then the brain edema was gradually decreased, and ultimately got right original level at 7d. Compared with the ICH group, the water content of brain in the rats of the intervention group was obviously decreased (P<0.05). The expression of CD14 in brain tissue cells of sham group was slight; CD14 protein began to express around hematoma in ICH group at 6h after operation, and a small number of positive cells increased with time, reached the peak at 72h, and then decreased gradually. The trend of TLR4 and IL-8 protein expression was consistent with that of CD14. The expression level of TLR4 and CD14 was positively correlated with brain water content (P<0.05), and the expression level of TLR4 and IL-8 was significantly decreased after Shenshao oral liquid. Conclusion: Shenshao oral liquid may reduce the inducing factors of CD14 / TLR4 Signal pathway, reduce the secondary inflammatory injury of brain tissue around hematoma after cerebral hemorrhage, and play a protective role in brain tissue.
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[1] 孟令丽,李楠,刘曼,等.大鼠脑出血模型的建立及评价[J].华北理工大学学报(医学版),2016(5):346~348.
[2] Lively S, Lyanne C, Schlichter. Age-related comparisons of evolution of the inflammatory response after intracerebral hemorrhage in rats [J]. Transl Stroke Res, 2012, 3(1): 132~146.
[3] Hua F, Ma J, Ha T. Activation of Toll-like receptor 4 signaling contribute to hippocampal neuronal death following global cerebral ischemia/reperfusion[J].Neuroimm, 2007, 190(1-2): 101~111.
[4] 杨永青,时斌武,杨利孙,等.血清IL-8、IL-10与急性脑出血患者血肿周围低密度的关系及醒脑静注射液对其血清学水平的影响[J].中国医药导刊,2016,18(12):1211~1212.
[5] 蒋令修,梁宇,陈文武,等.急性基底节脑出血IL-4、IL-6、IL-8及IL-10与周围脑组织水肿的相关性分析[J].中国现代医生,2015,53(34):25~27.
[6] 代艳楠,张春来,周红霞,等.参芍口服液对2型糖尿病心肌病大鼠心肌Toll样受体4表达的影响[J].重庆医学,2016,45(24):3327~3329,3333.
[7] 张琦,李映雪,尚小明,等.参芍口服液对动脉粥样硬化大鼠血脂和细胞黏附分子的影响[J].中国煤炭工业医学杂志,2017,20(9):1077~1080.
[8] Chunyan Lei,Bo Wu,Tian Cao,Ming Liu,Zilong Hao. Brain recovery mediated by toll-like receptor 4 in rats after intracerebral hemorrhage[J]. Brain Research,2016,1632.
[9] 郑永先,付斌,刘玉仁,等.TLR4在原发性脑出血患者外周血中的动态变化及意义[J].中国临床医生杂志,2018,46(5):553~556.
[10] 陶蕾,王高翔.TLR4及NF-kB在脑出血大鼠血肿周围组织的表达[J].贵州医科大学学报,2017,42(7):812~816. |
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2020, Vol. 26 
