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| Correlation between Survivin Acetylation and PCAF, P300 Expression in Esophageal Carcinoma and its Clinical Significance |
| ZHAO Baozhong, et al |
| Chengde Social Welfare Institute, Hebei Chengde 067000, China |
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Abstract Objective: To investigate the relationship between the acetylation level of Survivin protein and the expression of PCAF,P300 and the clinical features of esophageal carcinoma. Methods: The Survivin protein acetylation level were examined via co-immunoprecipitation experiment in 70 cases esophageal carcinoma tissue and peficancerous tissues. The PCAF and P300 protein were examined via Immunohistochemical staining experiment in esophageal carcinoma tissue.The relationship was analyzed between Survivin acetylation level and expression of PCAF, P300 and clinical case characteristics. Results: (1)The Survivin protein was acetylated in esophageal carcinoma tissue. The Survivin protein acetylation level in esophageal carcinoma tissue was 72.05±1.26%, it was obviously higher than the peficancerous tissues(13.03±1.34%). There was statistics difference (P<0.05);The Survivin protein acetylation level were closely related to esophageal carcinoma staging, differentiation and lymph node metastasis (P<0.05); (2) The positive expression of PCAF was 38.57% in cancer tissues,which was lower than that in normal tissues which was 87.14%. There was statistics difference (P<0.05); (3) The positive expression of P300 was 72.86%% in cancer tissues,which was higher than that in normal tissues which was 15.71%%. There was statistics difference (P<0.05); (4)The Survivin acetylation level and expression of PCAF in esophageal carcinoma was no correlation.The correlation coefficient r=0.2421 (P>0. 05);(5)The Survivin acetylation level and expression of P300 in esophageal carcinoma was interrelated.The correlation coefficient r=0.7453 (P<0. 05). Conclusions: Survivin protein acetylated in esophageal cancer tissue, and the occurrence and development of esophageal cancer may be closely related to the degree of acetylation of Survivin protein. The acetylation of Survivin protein was correlated with acetyltransferase P300, but not with PCAF.
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2019, Vol. 25 
