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| Screening Differentially Expressed Proteins in Serum of Patients with Contrast-induced Acute Kidney Injury by Proteomics Technology |
| GONG Yu, DING Feng, YANG Yan, et al |
| Shanghai Eighth People's Hospital, Shanghai 200235, China |
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Abstract Objective: To screen the differentially expressed proteins in the serum of patients with contrast-induced acute kidney injury (CI-AKI) after percutaneous coronary intervention (PCI) by proteomics technology. Methods: Serum samples were collected from 2 groups of patients: patients with AKI after PCI (CI-AKI patients group, n=10);patients without AKI after PCI (non-CI-AKI patients group, n=10). In order to study the dynamic alteration of serum proteome and screen for differentially expressed proteins in the course of disease progression, serum samples were collected from the two groups before exposure of contrast media (0 h) and 24 h and 48 h after the use of contrast media. Two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and bioinformatics analysis were performed. Results: In the CI-AKI patients group, serum proteome of patients with CI-AKI changed significantly after PCI. Apolipoprotein E was identified as the continuously differentially expressed protein (3-fold up-regulated) in the serum from 0 h to 24h and 0 h to 48 h after PCI. Conclusion: The expression profiles of serum proteins in CI-AKI patients changed significantly after PCI. Apolipoprotein E protein was continuously 3-fold up-regulated during the pathological process of CI-AKI, which may become a candidate serum protein marker with diagnostic value for CI-AKI.
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2019, Vol. 25 
