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| Inhibitory Effect and Mechanism of siRNA Targeting Nox2 on Adriamycin-induced Injury of Human Skin Fibroblasts |
| GUO Yadong, SUN Yaowen, QIN Gaoping, et al |
| Shaanxi People's Hospital, Shaanxi Xi'an 710000, China |
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Abstract Objective: To explore the effect of Nox2 in human dermal fibroblasts damage induced by adriamycin. Methods: A human dermal fibroblasts injury model was established by exposure to adriamycin. Western blotting was used to detect Nox2 protein expression in the injury of human dermal fibroblasts induced by adriamycin. The cell survival rate was determined by MTT method. Superoxide dismutase (SOD) activity, malondialdehyde (MDA) and reactive oxygen species (ROS) levels were detected by biochemical kit. Moreover, the level of apoptosis was detected by Western blotting.Results: The relative expression of Nox2 in adriamycin group was higher than that in the control group, and the difference was statistically significant (P<0.05). Compared with adriamycin group, inhibition of Nox2 expression by siRNA decreased the level of MDA and ROS, as well as enhanced the activity of SOD (P<0.05). In addition, inhibition of Nox2 expression by siRNA decreased the apoptosis of human dermal fibroblasts induced by adriamycin (P<0.05). Conclusion: Targeting inhibition of Nox2 expression by siRNA can protect against the injury of human dermal fibroblasts induced by adriamycin, the mechanism is the reducing of oxidative stress and ROS level.
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[1] 郭燕,张丽娜,顾林.新辅助化疗联合保乳手术可行性研究[J].肿瘤,2018,(3):242~249.
[2] Mclaughlin D, Zhao Y, O'Neill K M, et al. Signalling mechanisms underlying doxorubicin and Nox2 NADPH oxidase-induced cardiomyopathy: involvement of mitofusin-2[J]. Br Pharmacol,2017,174(21):3677~3695.
[3] 王震,王梦龙,刘剑芳,等.NADPH氧化酶在阿霉素心脏毒性的表达及意义[J].中国循证心血管医学杂志,2018(2):164~166.
[4] Woodley D T. Distinct Fibroblasts in the Papillary and Reticular Dermis: Implications for Wound Healing[J]. Dermatol Clin,2017,35(1):95~100.
[5] 杨新良,陈也君,胡高云,等.NADPH氧化酶及其抑制剂的研究进展[J].药学学报,2016,(4):499~506.
[6] Tsai M H, Lin Z C, Liang C J, et al. Eupafolin inhibits PGE2 production and COx2 expression in LPS-stimulated human dermal fibroblasts by blocking JNK/AP-1 and Nox2/p47(phox) pathway[J]. Toxicol Appl Pharmacol,2014,279(2):240~251.
[7] Kesik V, Yuksel R, Yigit N, et al. Ozone ameliorates doxorubicine-induced skin necrosis - results from an animal model[J]. Int Low Extrem Wounds,2016,15(3):248~254.
[8] Zhang G Y, Wu L C, Dai T, et al. NADPH oxidase-2 is a key regulator of human dermal fibroblasts: a potential therapeutic strategy for the treatment of skin fibrosis[J]. Exp Dermatol,2014,23(9):639~644. |
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2019, Vol. 25 
