has-miR-1-3p通过调控BDNF/TrkB信号通路抑制人滋养细胞增殖和侵袭

doi:10.3969/j.issn.1006-6233.2018.12.004

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Abstract Objective: To investigate the role and mechanism of has-miR-1-3p in proliferation and invasion of human trophoblast cell line HTR-8/Svneo. Methods: HTR-8/Svneo cells was transfected with has-miR-1-3p mimics as experimental group, and nonsense miRNAs were used as the control group. CCK8 and Transwell assays were used to detect the effects of has-miR-1-3p on cell proliferation and invasion. The target genes of has-miR-1-3p were predicted by an online analysis website. The regulation of has-Mi-1-3p on target genes was verified by dual luciferase report and Western blot assays. Results: has-miR-1-3p mimics significantly decreased cell proliferation and invasion of HTR-8/Svneo cells compared to the control cells. Moreover, the dual luciferase reporter and Western blot assays indicated that has-miR-1-3p mimics could directing target BDNF, and the phosphorylation level of TrkB was also inhibited. Furthermore, up-regulation of BDNF significantly reversed the inhibitory effects of has-miR-1-3p mimics on proliferation and invasion in HTR-8/Svneo cells. Conclusion: has-miR-1-3p inhibits proliferation and invasion of human trophoblast cells via regulating BDNF/TrkB pathway.

Key words： has-miR-1-3p Trophoblast cells Proliferation Invasion BDNF

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	XIAO Yanping
	FU Jiuyuan
	GE Yongmei
	et al

Cite this article:

XIAO Yanping,FU Jiuyuan,GE Yongmei, et al. has-miR-1-3p Inhibits Proliferation and Invasion of Human Trophoblast Cells Via Regulating BDNF/TrkB Pathway[J]. HeBei Med, 2018, 24(12): 1948-1952.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2018.12.004 OR http://www.hbyxzzs.cn/EN/Y2018/V24/I12/1948

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