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Effects of Laboratory Diagnosis of Hemolytic Disease of Newborn in Minjiang District |
LI Hongbing, RAN Bolin, WANG Chun, et al |
Chongqing Qianjiang Central Hospital, Chongqing 409000, China |
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Abstract Objective: To analyze the laboratory test results of hemolytic disease of newborn (HDN) in Minjiang District, and to study the clinical value of blood group distribution and hemolysis three tests in the diagnosis of HDN. Methods: A total of 1,147 suspected HDN samples were collected from the Department of Obstetrics and Pediatric Department of Minjiang Central Hospital from August 2012 to August 2016. The hemolysis three tests, namely, direct antiglobulin test (DAT), free antibody test (FAT) and antibody released test (ART) were performed by the micro-column gel technique, and results were analyzed. Results: Of the 1,147 samples, there were 788 cases (68.70%) of maternal- fetal blood group incompatibility, 481 cases of positive HDN and the positive rate was 61.04% (481/788). Results of hemolysis three tests of 481 cases with HDN showed that the positive rates of DAT, FAT and ART were 28.27% (136/481), 86.90% (418/481) and 100%, respectively. Compared with the other three kinds of combination, the positive rate of FAT+ART was significantly higher (P<0.05). In 788 specimens of blood group incompatibility, the positive rate of ABO-HDN was significantly higher than that of Rh-HDN [73.01% (468/641) vs 8.84% (13/147)] (P<0.05). The positive rate of type A HDN was 81.46% (246/302) in 641 ABO blood group incompatibility specimens, which was significantly higher than that of type B HDN [66.08% (224/339)] (P<0.05). In 147 Rh blood group incompatible specimens, the positive rate of Rh D-HDN was 16.18% (11/68), which was significantly higher than Rh E-HDN positive rate [3.92% (2/51)] or Rh C-HDN positive rate [0% (0/28)] (P<0.05). Conclusion: In the hemolysis three tests, the sensitivity of ART is the highest, which is the strongest evidence for the diagnosis of HDN. The incidence of HDN is significantly higher in mothers and infants with ABO blood group incompatibility than in those with Rh blood group incompatibility, and type A is significantly higher than type B in ABO blood group system. Rh D is the highest in Rh blood group system.
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[1] 高艳霞,王俊芳,刘静兰.孕妇ABO血型免疫性抗体效价与新生儿溶血病的相关性分析[J].检验医学,2016,31(1):76~77. [2] 毛中华,王维娜,邢志勇,等.新生儿溶血病与O型孕妇血清IgG抗体的相关性分析[J].中国输血杂志,2016,29(5):503~504. [3] 张毅,崔吉香,李慧,等.微柱凝胶技术在O型孕妇IgG抗A/B效价与ABO新生儿溶血病相关性研究中的应用[J].检验医学与临床,2016,13(12):1626~1627. [4] 赵倩,王振雷,何路军,等.Rh血型系统意外抗体致新生儿溶血病的实验室分析[J].河北医学,2018,24(2):283~286. [5] 李小红.血型鉴定及不规则抗体筛查与新生儿溶血病相关性分析[J].中国基层医药,2017,24(6):934~936. [6] 阳志勇,孙涛.抗体筛查在门诊产科常规检测中预防HDN的应用价值--附1例抗-M引起的新生儿溶血病[J].中国免疫学杂志,2017,33(2):278~279. [7] Sarper N,Zengin E,Jakobs C,et al.Mild hemolytic anemia,progressive neuromotor retardation and fatal outcome:a disorder of glycolysis,triose-phosphate isomerase deficiency.[J].Turkish Journal of Pediatrics,2013,55(3):198~202. [8] 杨冬梅,李志坚.ABO新生儿溶血病引起高胆红素血症患儿溶血三项试验的相关性研究[J].中国药物与临床,2017,17(6):911~913. [9] 叶海辉,黄洪海,王晓霖,等.孕妇IgG效价与不同血型新生儿溶血并发症的相关性分析[J].中国实验血液学杂志,2017,25(5):1532~1536. [10] 贺坤华,马丽琼,崔会芬,等.高效价Rh系统抗体致新生儿溶血病研究[J].国际检验医学杂志,2016,37(5):654~656. [11] 田晓妍.微柱凝胶技术对ABO新生儿溶血病患儿输血前检验效果的影响分析[J].临床检验杂志(电子版),2017,6(3):441~442. [12] 方声,黄邀,卢洪萍,等.脐带血血清总胆红素检测在新生儿溶血病早期诊治中的应用价值[J].实用预防医学,2017,24(4):491~494. |
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