|
|
|
| Changes of Platelet Mitochondrial Function in Asthmatic Patients |
| LIU Huifang, MA Hongxia |
| Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Xinjiang Urumqi 830000, China |
|
|
|
|
Abstract Objective: To investigate the changes of platelet mitochondrial function in asthmatic patients. Methods: 122 cases were diagnosed as acute episode of asthma from April 2016 to May 2017. According to the severity grading standard, they were divided into 4 groups, 45 cases of intermittent state (first grade), 23 cases of mild asthma (second grade), 30 cases of moderate asthma (third grade) and 24 cases of severe asthma (fourth grade). 44 cases of normal control group came from the physical examination center at same time. Platelet and platelet mitochondria were separated from blood samples. The ultrastructure of platelets was observed by transmission electron microscopy. The platelet mitochondrial membrane potential (MMP), cytochrome C oxidase (COX) activity and adenosine triphosphate (ATP) activity were detected and their differences were analyzed. Results: There were significant differences in platelet mitochondrial membrane potential (MMP), cytochrome C oxidase (COX) activity and adenosine triphosphate (ATP) between asthma group and normal group (P<0.05). The mitochondrial function (MMP, ATP, COX) of asthmatic patients with different severity was significantly different from that of normal control group (P<0.05). Conclusion: There are mitochondrial respiratory chain inhibition and impaired function in asthmatic patients. Mitochondrial damage and dysfunction may play an important role in the pathogenesis of asthma.
|
|
|
|
|
|
[1] 赵彩霞.支气管哮喘发病机制的研究进展[J].医学理论与实践,2016,29(7):870~872.
[2] Kleniewska P, Pawliczak R.The participation of oxidative stress in the patho genesis of bronchial asthma[J].Biomedicine & pharmacotherapy,2017, 94: 100.
[3] Palmieri F, Pierri C L, De G A, et al. Evolution, structure and function of mitochondrial carriers: a review with new insights[J]. Plant Journal,2011, 66(1):161~181.
[4] Yk D, Novgorodtseva T, Kondrat'Eva E V, et al. Morpho-functional characteristics of blood cell mitochondria in bronchial asthma[J]. Klinicheskaia Meditsina,2015, 93(10): 47.
[5] 王蕾,李锋,周新.线粒体与慢性气道疾病[J].国际呼吸杂志,2017,37(19):1511~1514.
[6] Garcia-Souza LF, Oliveira MF. Mitochondria: biological roles in platelet physiology and pathology[J].Int Biochem Cell Biol,2014,50:156~160.
[7] 中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南(2016年版)[J].中华结核和呼吸杂志,2016,9(9):675~697. |
|
|
|
2018, Vol. 24 
