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| Laboratory Analysis of Irregular Antibodies in Rh Blood Group System Causing Hemolytic Disease of The Newborn |
| ZHAO Qian, WANG Zhenlei, HE Lujun, et al |
| Hebei Blood Center, Hebei Shijiazhuang 050000, China |
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Abstract Objective: To analyze the distribution of all kinds of irregular antibodies causing hemolytic disease of the newborn (HDN) in Rh blood group system to provide important basis for clinical prevention, diagnosis and the treatment of Rh-HDN. Methods: The ABO and Rh blood group identification (neonatal ABO blood group identification was done only by positive test) by the tube method. Three tests of neonatal hemolysis were carried out by microcolumn gel method. The antibody screening and antibody identification were carried out by microcolumn gel method and polyamine method. The application of 2 test were compared between the two groups. Result: In 246 cases of compatible ABO blood type of the parents, there were 61 cases of irregular antibodies detected in Rh blood group system (24.60%), and the distribution of antibodies were anti-D(32 cases), anti-E(2 cases), anti-cE(4 cases), anti-C(3 cases), anti-c(2 cases) and anti-Ce(4 cases) respectively. In 273 cases of incompatible ABO blood type of the parents, there were 25 cases of irregular antibodies detected in Rh blood group system (8.93%), and the distribution of antibodies were anti-D(15 cases), anti-E(2 cases), anti-cE(4 cases), anti-C(2 cases), anti-c(1 case) and anti-Ce(1 case) respectively. The detected number of Rh antibodies in the group without ABO antibodies was higher than the number of the group with ABO antibodies. The differences was statistically significant (P＜0.05). Conclusion: Anti-D is still the main cause of Rh-HDN. However, the detection rates of anti -E and anti-cE were also high in other Rh systems, especially in the samples of the same or compatible ABO blood group. We should attach importance to the RhD positive pregnant women. If the ABO blood group is the same or coincide, the screening of unexpected antibodies in Rh blood group system is of great clinical significance for the prevention and early treatment of Rh-HDN.
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2018, Vol. 24 
