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| The Role and Mechanism of LGR5 in Cervical Carcinogenesis |
| CHEN Qing, LI Xianghong, ZHAO Jinyan, et al |
| The Second Affiliated Hospital of Xi'an Jiaotong University, Shannxi Xi'an 710004, China |
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Abstract Objective: To investigate the role and mechanism of LGR5 in cervical carcinogenesis. Methods: The expression of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and squamous cervical cancer were determined by immunohistochemistry and western blot, and analyze the relationship between LGR5 and clinical pathological features of cervical cancer and β-catenin expression. The effect of LGR5 on cervical carcinogenesis was examined using tumorsphere formation. Results: The positive rate of LGR5 and β-catenin in normal cervix, cervical intraepithelial neoplasias and cervical cancer were 17% (5/30), 65% (11/17), 84% (54/64) and 13%(4/30),53%(9/17),92%(59/64),respectively. The difference was statistically significant (P<0.01). The expression of LGR5 was associated with pathological grade of cervical cancer, but not with patient's age, clinical stage and lymph node metastasis. β-catenin expression in cervical cancer was positively related to LGR5 expression(r=0.69, P<0.01). Moreover, the rate of tumorsphere formation of LGR5 overexpression SiHa cell was significantly higher than the GFP control group (P<0.01). Conclusion: LGR5 and promoted cervical carcinogenesis and its mechanismmay be associated with high expressio0n of β--catenin
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2017, Vol. 23 
