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The Relationship between MICA Gene Expression of Bladder Cancer and Mutation of p53 and K-ras Gene |
GU Jingfeng, WANG Guiqi, et al |
The First Hospital of Hebei Medical University, Hebei Shijiazhuang 050031, China |
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Abstract Objective: To investigate the relationship between the expression of MICA gene in bladder cancer and the mutation of p53 gene and K-ras gene. Methods: 66 cases of bladder cancer patients with clinical data in our hospital from March 2014 to March 2016 were analyzed. Semi quantitative PCR-SSCP technique was used to detect the expression of MDR1 gene and p53 and K-ras in cancer tissues, and analyzed the relationship between the expression of MICA gene in colorectal cancer and the mutation of p53 and K-ras genes. Results: The study found that there was no correlation between the expression of MICA and the location, age and sex of the patients with colorectal cancer (P > 0.05). In 66 patients, the mean expression of MICA in 22 lymph node metastasis patients was higher than that without lymph node metastasis, and the difference was statistically significant (P < 0.05). 8 cases of K-ras and p53 gene expression of the MICA gene mutation combined with average weight is higher than non mutation, the difference was statistically significant (P < 0.05), 12 cases of K-ras gene mutation MICA average expression was slightly higher than that without mutation, but the difference was not statistically significant (P > 0.05), 16 cases of p53 gene mutation MICA expression was significantly better than the average mutation in the p53 gene, the difference was statistically significant (P < 0.05). Conclusion: The high expression of MICA gene has become a key indicator for the detection of bladder cancer, and the correlation between K-ras and p53 gene and its high expression.
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