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| Effects of Simvastatin on OFR, NO and NOS in Brain Tissue of Rats with Renal Ischemia Reperfusion Injury |
| JING Jiao, ZHANG Yongzhong, YAN Wensheng, et al |
| Shijiazhuang Medical College, Hebei Shijiazhuang 050000, China |
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Abstract Objective: To observe the effect of simvastatin on brain tissue after renal ischemia reperfusion injury and its mechanism. Methods: A rat model of renal ischemia reperfusion injury (RIRI) was prepared by clamping the bilateral renal arteries for 45 minutes(10% chloral hydrate intraperitoneal anesthesia, open the abdominal cavity, exposed bilateral renal artery and vein, clamping the bilateral renal artery and vein with artery clamp, 45min after removal of the artery clamp to restore blood flow, and then suture the abdominal wall). 36 rats were randomly divided into the following groups, 12 rats in each group: sham operation group; renal ischemia reperfusion group; simvastatin group. Detection of serum creatinine (Scr), urea nitrogen (BUN), brain tissue malondialdehyde (MDA) and nitric oxide (NO) content, superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) activity in brain tissue was detected in endothelial nitric oxide synthase (eNOS) protein expression level. Results: Compared with the sham operation group, the contents of Scr, BUN, MDA, NO and iNOS activity in the ischemic group increased (P<0.05), while the activity of SOD decreased (P<0.05). Compared with the ischemia reperfusion group, the content of SCr, BUN and MDA decreased, iNOS activity was decreased (P<0.05), but the activity of SOD and the content of NO increased (P<0.05). The content of eNOS protein in renal ischemia reperfusion group was higher than that in sham operation group (P<0.05), and the content of eNOS protein in simvastatin group was higher than that in the renal ischemia reperfusion injury group (P<0.05). Conclusion: Simvastatin can protect the brain from ischemia reperfusion injury. It may be related to decreased the activity of iNOS, and increase the expression of eNOS protein and the content of NO.
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2017, Vol. 23 
