Abstract：Objective: To analysis the effect of Buguzhi Decoction on Wnt/PPARγ signal pathway in healing process of fracture in type 2 diabetic rats. Methods: Seventy-two healthy male SD rats were randomly divided into control group, model group, sodium fluoride and Buguzhi group, each with 18 rats. The tibial fracture model was established in control group, type 2 diabetic tibial fracture model were established by intraperitoneal injection of streptozotocin in the other groups, and were given corresponding drug treatment. The tibia BMD, FPG and serum Ca、P、ALP levels, tibia bending strength and torque, the expression levels of β-catenin, p-GSK3β and PPARγ in bone tissue were detected. Results: After treatment, compared with model control group, the tibia BMD levels were higher in rats of each group,and Buguzhi group was higher than sodium fluoride group(P<0.05);the serum Ca、P levels were lower in rats of each group,and Buguzhi group was lower than sodium fluoride group (P<0.05);the serum ALP levels were higher in rats of each group,and Buguzhi group was higher than sodium fluoride group (P<0.05);the tibia bending strength and torque levels were higher in rats of each group,and Buguzhi group was higher than sodium fluoride group (P<0.05);the expression of β-catenin protein in bone tissue of rats in each group was higher in rats of each group, and Buguzhi group was higher than sodium fluoride group (P<0.05);the expression of p-GSK3β protein in bone tissue of rats in each group was lower in rats of each group, and Buguzhi group was lower than sodium fluoride group (P<0.05);the expression of PPARγ protein in bone tissue of rats in each group was lower in rats of each group, and Buguzhi group was lower than sodium fluoride group (P<0.05). Conclusions: Buguzhi Decoction can effectively improve the BMD、serum ALP levels of fracture and type 2 diabetic rats, reduce serum Ca、P levels, improve the biomechanical strength of bone, accelerate fracture healing, it may be associated with up regulation ofβ-catenin protein expression, down regulation of p-GSK3β、PPARγ protein expression, activation of the Wnt signaling pathway and inhibition of the PPAR signaling pathway
曹大宁, 阳春华, 李聪. 补骨脂煎剂对2型糖尿病大鼠骨折愈合过程中Wnt/PPARγ信号通路影响研究[J]. 河北医学, 2017, 23(4): 651-655.
CAO Daning, YANG Chunhua, LI Cong. Study on the Effect of Buguzhi Decoction on Wnt/PPARγ Signal Pathway in Healing Process of Fracture in Type 2 Diabetic Rats. 河北医学, 2017, 23(4): 651-655.
[1] 孙贤杰.骨折愈合的影响因素[J].中国医药指南,2012,10(17):82~84. [2] 盛华刚.影响骨折愈合因素的研究进展[J].北方药学,2013,10(3):53. [3] 刘建恒,张里程,唐佩福.骨折延迟愈合和不愈合的诊治现状[J].中华外科杂志,2015,53(6):464~467. [4] Wang C Y,Yang H B,Hsu H S,et al.Mesenchymal stem cell-conditioned medium facilitates angiogenesis and fracture healing in diabetic rats[J].Journal of Tissue Engineering&Regenerative Medicine,2012,6(7):559~569. [5] 李凯明,郝延科.补骨脂抗骨质疏松症的研究进展[J].中医药临床杂志,2016,28(6):886-889. [6] 刘振东,刘亚江,高敏伟,等.2型糖尿病大鼠骨折愈合障碍与体内晚期糖基化终末产物的变化[J].中国组织工程研究,2014,18(20):3122~3126. [7] 李溪,向盈盈,龚跃昆,等.糖尿病大鼠骨折愈合早期局部骨痂组织中Ⅰ型胶原纤维表达和骨密度的变化[J].中国组织工程研究,2012,16(33):6097~6101. [8] Stolzing A,Sellers D,Llewelyn O,et al.Diabetes induced changes in rat mesenchymal stem cells[J].Cells Tissues Organs,2010,191(6):453. [9] Parizi A M,Jelodar G,Moslemi H,et al.Influence of hydroxyapatite on fracture healing in diabetic rats:biomechanical and radiographic studies[J].Veterinary Archives,2010,80(1):113~120. [10] 柴丽娟,张晗,王少峡,等.中药补骨脂的药理作用研究进展[J].海峡药学,2013,25(7):12~14.
2017, Vol. 23 
冀公网安备13080202000786号