Abstract：Objective: To investigate the effect and mechanism of different concentrations Ophiopogonin-B (OP-B) on the malignant behavior of breast cancer cells. Methods: Human breast cancer cells were inducted into control group, OP-B low, medium and high concentration groups (transfected with 10, 20 and 40 μmol/L OP-B), high concentration OP-B+pcDNA-NC group (transfected with pcDNA-NC and 40 μmol/L OP-B ), and high concentration OP-B+pcDNA-PD-1 group (transfected with pcDNA-PD-1 and 40 μmol/L OP-B ). The proliferation ability, the migration and invasion abilities, and the apoptosis rate of MCF-7 cells were detected by cell counting kit-8 (CCK-8) assay, Transwell and Flow cytometry, respectively. Western blot was used to detect the protein expression levels of programmed death-1 (PD-1), programmed death-ligand 1 (PD-L1), vascular endothelial growth factor (VEGF), and transforming growth factor beta1 (TGF-β1) of MCF-7 cells in each group. Results: Compared with the control group, the proliferation rate, migration and invasion ability of MCF-7 cells in each treatment group of OP-B were significantly reduced (P<0.05), and the apoptosis rate was significantly increased (P<0.05), the protein expression levels of PD-1, PD-L1, VEGF and TGF-β1 in MCF-7 cells were significantly reduced (P<0.05). OP-B could significantly inhibit the malignant behavior and immune escape of MCF-7 cells, showing a concentration dependent (P<0.05), however, pcDNA-PD-1 plasmid transfection could significantly reverse the effect of high concentration OP-B on MCF-7 cells (P<0.05). Conclusion: OP-B may reduce the expression of VEGF and TGF-β1 by inhibiting the PD-1/PD-L1 pathway, thereby reducing proliferation, migration, and invasion of MCF-7 cell, promoting cell apoptosis, and reducing the occurrence of immune escape.
李世芬, 蒋建平, 陈红. 麦冬皂苷B调节PD-1/PD-L1信号通路对乳腺癌细胞恶性进展的影响[J]. 河北医学, 2025, 31(2): 192-197.
LI Shifen, JIANG Jianping, CHEN Hong. Effect of Ophiopogonin-B on Malignant Progression of Breast Cancer Cells by Regulating PD-1/PD-L1 Signaling Pathway. HeBei Med, 2025, 31(2): 192-197.
[1] Nikkhoo A,Rostami N,Hojjat-farsangi M,et al.Smac mimetics as novel promising modulators of apoptosis in the treatment of breast cancer[J].Cell Biochem,2019,120(6):9300-9314. [2] Haji-fatahaliha M,Hosseini M,Akbarian A,et al.CAR-modified T-cell therapy for cancer:an updated review[J].Artif Cells Nanomed Biotechnol,2016,44(6):1339-1349. [3] Topper MJ,Vaz M,Marrone KA,et al.The emerging role of epigenetic therapeutics in immuno-oncology[J].Nat Rev Clin Oncol,2020,17(2):75-90. [4] Bastaki S,Irandoust M,Ahmadi A,et al.PD-L1/PD-1 axis as a potent therapeutic target in breast cancer[J].Life Sci,2020,15(247):117437. [5] 骆晴,刘明伟.三白草酮调节PD-1/PD-L1信号通路对食管癌细胞恶性生物学行为及免疫抑制的影响[J].中国细胞生物学学报,2024,46(7):1442-1449. [6] Zhang S,Li H,Li L,et al.Ophiopogonin B inhibits migration and invasion in non-small cell lung cancer cells through enhancing the interaction between Axin and β-catenin[J].Cancer,2021,12(20):6274-6284. [7] Yuan S,Xu Y,Yi T,et al.The anti-tumor effect of OP-B on ovarian cancer in vitro and in vivo,and its mechanism:an investigation using network pharmacology-based analysis[J].Ethnopharmacol,2022,283(1):114706. [8] Xia C,Dong X,Li H,et al.Cancer statistics in China and United States,2022:profiles,trends,and determinants[J].Chin Med (Engl),2022,135(5):584-590. [9] Zhang L,Li C,Zhang Y,et al.Ophiopogonin B induces gastric cancer cell death by blocking the GPX4/xCT-dependent ferroptosis pathway[J].Oncol Lett,2022,23(3):104. [10] 李丽秋,高倩,顾玲,等.麦冬皂苷B通过调控miR-432-5p抑制A549细胞增殖、迁移和侵袭[J].中国药理学通报,2021,37(7):1035-1036. [11] Syamsu SA,Faruk M,Smaradania N,et al.PD-1/PD-L1 pathway:current research in breast cancer[J].Breast Dis,2024,43(1):79-92. [12] Zhang Y,Brekken RA.Direct and indirect regulation of the tumor immune microenvironment by VEGF[J].Leukoc Biol,2022,111(6):1269-1286.
2025, Vol. 31 
冀公网安备13080202000786号