外源性神经生长因子激活Wnt3α/β-catenin信号通路促进骨折愈合

doi:10.3969/j.issn.1006-6233.2024.10.08

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摘要 目的:分析外源性神经生长因子激活Wnt3α/β-catenin信号通路促进骨折愈合的作用。方法:选取40只SPF级SD大鼠,10只大鼠作为对照组,30只构建胫骨骨折模型,共有27只大鼠建模成功,分为模型组9只、低剂量组9只、高剂量组9只。观察各组大鼠病理组织学、骨钙、骨磷水平、血液流变学指标、BMD、骨痂直径、血管生成相关因子、炎症因子水平、Wnt3α/β-catenin通路关键基因Wnt3a、β-catenin、GSK-3β的mRNA表达量、Wnt3α/β-catenin通路关键蛋白Wnt3a、β-catenin、GSK-3β的表达。结果:与对照组相比,另外三组骨钙、骨磷、BMD、血管生成相关因子、Wnt3α、β-catenin相关mRNA表达量、Wnt3α、β-catenin表达量降低,全血黏度、血浆黏度、炎症因子水平、GSK-3β相关mRNA表达量、GSK-3β表达量升高,与模型组相比,低、高剂量组骨钙、骨磷、BMD、骨痂直径、血管生成相关因子、Wnt3α、β-catenin相关mRNA表达量、Wnt3α、β-catenin表达量升高,全血黏度、血浆黏度、炎症因子水平、GSK-3β相关mRNA表达量、GSK-3β表达量降低;且高剂量组体现出最高骨钙、骨磷、BMD、骨痂直径、血管生成相关因子、Wnt3α、β-catenin相关mRNA表达量、Wnt3α、β-catenin表达量,最低全血黏度、血浆黏度、炎症因子水平、GSK-3β相关mRNA表达量、GSK-3β表达量(P<0.05)。结论:采用外源性神经生长因子对胫骨骨质大鼠干预,可促使大鼠骨折的愈合,其作用机制可能与激活Wnt3α/β-catenin信号通路有关,具有较好的使用效果。

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	赵研哲
	刘建
	石晓云

关键词 ：胫骨骨折, 外源性神经生长因子, Wnt-3α/β-连环蛋白

Abstract：Objective: To analyze the effect of exogenous nerve growth factor on the activation of Wnt3α/β-catenin signaling pathway in promoting fracture healing. Methods: Select 40 SPF grade SD rats, 10 rats as the control group, and 30 rats to construct a tibial fracture model. A total of 27 rats were successfully modeled and divided into a model group of 9 rats, a low-dose group of 9 rats, and a high-dose group of 9 rats. Observe the pathological histology, bone calcium and phosphorus levels, hemorheological indicators, BMD, callus diameter, angiogenesis related factors, inflammatory factor levels, mRNA expression levels of key genes Wn3a, β -catenin, and GSK-3β in the Wnt3 α/β-catenin pathway, and expression levels of key proteins Wnt3a, β-catenin, and GSK-3β in the Wnt3 α/β-catenin pathway in each group of rats. Results: Compared with the control group, the other three groups showed a decrease in bone calcium, bone phosphorus, BMD, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and an increase in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β related mRNA expression, and GSK-3β expression. Compared with the model group, the low and high dose groups showed an increase in bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3 α, β - catenin related mRNA expression, Wnt3α, β-catenin expression, and a decrease in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3 β related mRNA expression, and GSK-3β expression; And the high-dose group showed the highest levels of bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and the lowest levels of whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β - related mRNA expression, and GSK-3β expression (P<0.05). Conclusion: The intervention of exogenous nerve growth factor on tibial bone can promote the healing of fracture in rats, the mechanism of which may be related to the activation of Wnt3α/β-catenin signaling pathway, and has a good effect.

Key words： Tibia fracture Exogenous nerve growth factor Wnt-3α/ β-catenin

基金资助:河北省石家庄市科学技术研究与发展自筹计划,(编号:221200313)

通讯作者: 石晓云

引用本文:

赵研哲, 刘建, 石晓云. 外源性神经生长因子激活Wnt3α/β-catenin信号通路促进骨折愈合[J]. 河北医学, 2024, 30(10): 1627-1633.
ZHAO Yanzhe, LIU Jian, SHI Xiaoyun. Exogenous Nerve Growth Factor Activates the Wnt3α/β-Catenin Signaling Pathway to Promote Fracture Healing. HeBei Med, 2024, 30(10): 1627-1633.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.10.08 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I10/1627

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