跨膜蛋白Klotho与纤连蛋白在瘢痕疙瘩表达水平及对术后复发的相关性分析

doi:10.3969/j.issn.1006-6233.2024.08.018

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摘要 目的: 探究Klotho蛋白结合纤连蛋白(FN)在瘢痕疙瘩术后复发的相关性。方法: 选取2020年4月至2023年4月于我科就诊的耳部瘢痕疙瘩患者43例及普通求美者37例,实验对象分为研究组(瘢痕疙瘩组织和血清)及对照组(正常组织和血清)。采用免疫组织化学染色法及酶联免疫吸附法(ELISA)检测比较两组组织和血清标本中Klotho蛋白及上皮间质转化标志物纤连蛋白FN的表达。同时在术后1月、3月、6月、12月对瘢痕疙瘩患者进行随访、记录复发情况,分为复发组及未复发组。应用统计学方法分析Klotho的表达与瘢痕疙瘩纤维化标志物FN的表达及术后复发的相关性。结果: 免疫组化结果:研究组Klotho阳性率较对照组低(P<0.05),为37.20%;研究组FN阳性率为72.10%,高于对照组阳性率(P<0.05);复发组Klotho阳性率为21.40%,低于未复发组(P<0.01);复发组FN阳性率为92.90%,高于未复发组(P<0.05)。Klotho和FN两种蛋白水平的相关性存在统计学差异(r=-0.307,P<0.05 );ELISA检测血清结果:研究组Klotho表达含量为65.99(55.05,79.06)ng/mL,低于对照组(P<0.05);研究组FN表达含量为83.56(62.67,91.85) ng/mL,较高于对照组(P<0.01);经Logrank检验,在术后复发患者中Klotho阴性表达与FN蛋白阳性表达相关(P均<0.05)。结论: Klotho蛋白结合上皮间质转化标志物FN可作为预测瘢痕疙瘩术后复发的重要标志物。

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关键词 ： Klotho蛋白, 上皮间质转化, 纤连蛋白, 瘢痕疙瘩

Abstract：Objective: To investigate the correlation between Klotho protein binding to fibronectin Fn and postoperative recurrence in keloids.Methods: A total of 43 patients with ear keloids and 37 general cosmetic seekers who visited our department from April 2020 to April 2023 were selected. The subjects were divided into a study group (keloid tissues and serum) and a control group (normal tissues and serum). Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect and compare the expression of Klotho protein and the epithelial-mesenchymal transition marker fibronectin FN in tissue and serum samples from both groups. Keloid patients were followed up at 1 month, 3 months, 6 months, and 12 months postoperatively to record recurrence, which divided them into recurrence and non-recurrence groups. Statistical methods were used to analyze the correlation between Klotho expression and fibronectin FN expression and postoperative recurrence in keloids.Results: Immunohistochemistry results showed that the positive rate of Klotho in the study group was lower than in the control group (P<0.05), at 37.20%. The positive rate of FN in the study group was 72.10%, higher than in the control group (P<0.05). The positive rate of Klotho in the recurrence group was 21.40%, lower than in the non-recurrence group (P<0.01), and the positive rate of FN in the recurrence group was 92.90%, higher than in the non-recurrence group (P<0.05). There was a statistically significant correlation between the levels of Klotho and FN proteins (r=-0.307, P<0.05). Elisa results showed that the expression level of Klotho in the study group was 65.99 (55.05,79.06) ng/mL, lower than in the control group (P<0.05), and the expression level of FN in the study group was 83.56 (62.67,91.85) ng/mL, higher than in the control group (P<0.01). Logrank tests indicated that negative Klotho expression and positive FN protein expression were associated with postoperative recurrence (both P<0.05).Conclusion: The combination of Klotho protein and the epithelial-mesenchymal transition marker FN can be used as an important marker to predict postoperative recurrence of keloids.

Key words： Klotho protein Epithelial-mesenchymal transition Fibronectin Keloids

基金资助:河北省承德市科学技术研究与发展计划项目,(编号:202201A111)

通讯作者: 郭有新

引用本文:

孙艳萍, 刘鹏超, 胡金宏, 梁亚坤, 韩福生, 唐海龙, 缪国东, 马志红, 郭有新. 跨膜蛋白Klotho与纤连蛋白在瘢痕疙瘩表达水平及对术后复发的相关性分析[J]. 河北医学, 2024, 30(8): 1331-1336.
SUN Yanping, LIU Pengchao, HU Jinhong, et al. Correlation Analysis of the Expression Levels of Transmembrane Protein Klotho and Fibronectin in Keloids and Their Relationship with Postoperative Recurrence. HeBei Med, 2024, 30(8): 1331-1336.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.08.018 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I8/1331

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