Abstract：Objective: To investigate whether circ-RBM33 can target the miR-383-3p/CKS1B axis to inhibit the proliferation and invasion of nasopharyngeal carcinoma cells.Methods: qRT-PCR was used to detect the expression of circ-RBM33 and miR-383-3p in CNE1 and HNEpC cells. CNE1 cells were randomly divided into si-NC group, si-RBM33 group, miR-NC group, miR-383-3p group, and si-RBM33 + anti-miR-383-3p group. Cell proliferation (CCK-8 assay), invasion (Transwell assay), migration (scratch assay), and expression of CKS1B protein in cells (Western blot) were detected. Luciferase activity assay was performed to determine the targeting relationship between circ-RBM33 and miR-383-3p/CKS1B.Results: The relative expression of circ-RBM33 in human nasopharyngeal carcinoma CNE1 cells was higher than that in human nasopharyngeal mucosal epithelial HNEpC cells, while the relative expression of miR-383-3p was lower than that in HNEpC cells (P<0.05). In the si-RBM33 group, the relative expression of circ-RBM33 in CNE1 cells, cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were lower than those in the si-NC group (P<0.05). In the si-RBM33 + anti-miR-383-3p group, the cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were higher than those in the si-RBM33 group (P<0.05). In the miR-383-3p group, the relative expression of miR-383-3p in CNE1 cells was significantly increased, while cell viability, number of invading cells, scratch closure rate, and expression of CKS1B protein in cells were lower than those in the miR-NC group (P<0.05). Transfection with WT-circ-RBM33/CKS1B resulted in significantly lower luciferase activity in the miR-383-3p group than in the miR-NC group (P<0.0001).Conclusion: Knockdown of circ-RBM33 may inhibit the proliferation, invasion, and migration of nasopharyngeal carcinoma cells by upregulating miR-383-3p expression and subsequently suppressing CKS1B expression.
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2024, Vol. 30 
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